Loss of the polycomb protein Mel-18 enhances the epithelial-mesenchymal transition by ZEB1 and ZEB2 expression through the downregulation of miR-205 in breast cancer
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, J-Y | - |
dc.contributor.author | Park, M. K. | - |
dc.contributor.author | Park, J-H | - |
dc.contributor.author | Lee, H. J. | - |
dc.contributor.author | Shin, D. H. | - |
dc.contributor.author | Kang, Y. | - |
dc.contributor.author | Lee, C. H. | - |
dc.contributor.author | Kong, G. | - |
dc.date.accessioned | 2022-07-16T05:45:49Z | - |
dc.date.available | 2022-07-16T05:45:49Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2014-03 | - |
dc.identifier.issn | 0950-9232 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/160544 | - |
dc.description.abstract | The epithelial-mesenchymal transition (EMT) is the pivotal mechanism underlying the initiation of cancer invasion and metastasis. Although Mel-18 has been implicated in several biological processes in cancer, its function in the EMT of human cancers has not yet been studied. Here, we demonstrate that Mel-18 negatively regulates the EMT by epigenetically modulating miR-205. We identified miR-205 as a novel target of Mel-18 using a microRNA microarray analysis and found that Mel-18 increased miR-205 transcription by the inhibition of DNA methyltransferase-mediated DNA methylation of the miR-205 promoter, thereby downregulating its target genes, ZEB1 and ZEB2. Furthermore, the loss of Mel-18 promoted ZEB1- and ZEB2-mediated downregulation of E-cadherin transcription and also enhanced the expression of mesenchymal markers, leading to increased migration and invasion in MCF-7 cells. In MDA-MB-231 cells, Mel-18 overexpression restored E-cadherin expression, resulting in reduced migration and invasion. These effects were reversed by miR-205 overexpression or inhibition. A tumor xenograft with Mel-18 knockdown MCF-7 cells consistently showed increased ZEB1 and ZEB2 expression and decreased E-cadherin expression. Taken together, these results suggest that Mel-18 functions as a tumor suppressor by its novel negative control of the EMT, achieved through regulating the expression of miR-205 and its target genes, ZEB1 and ZEB2. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | Loss of the polycomb protein Mel-18 enhances the epithelial-mesenchymal transition by ZEB1 and ZEB2 expression through the downregulation of miR-205 in breast cancer | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, J-Y | - |
dc.contributor.affiliatedAuthor | Kong, G. | - |
dc.identifier.doi | 10.1038/onc.2013.53 | - |
dc.identifier.scopusid | 2-s2.0-84895924719 | - |
dc.identifier.wosid | 000332631100013 | - |
dc.identifier.bibliographicCitation | ONCOGENE, v.33, no.10, pp.1325 - 1335 | - |
dc.relation.isPartOf | ONCOGENE | - |
dc.citation.title | ONCOGENE | - |
dc.citation.volume | 33 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 1325 | - |
dc.citation.endPage | 1335 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Genetics & Heredity | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Genetics & Heredity | - |
dc.subject.keywordPlus | GROWTH-FACTOR-BETA | - |
dc.subject.keywordPlus | TUMOR INVASION | - |
dc.subject.keywordPlus | MICRORNAS | - |
dc.subject.keywordPlus | BMI-1 | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | OVEREXPRESSION | - |
dc.subject.keywordPlus | REPRESSION | - |
dc.subject.keywordPlus | FAMILY | - |
dc.subject.keywordAuthor | Mel-18 | - |
dc.subject.keywordAuthor | miR-205 | - |
dc.subject.keywordAuthor | ZEB1 | - |
dc.subject.keywordAuthor | ZEB2 | - |
dc.subject.keywordAuthor | epithelial-mesenchymal transition | - |
dc.subject.keywordAuthor | polycomb | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.