Kaempferol inhibits IL‑1β‑induced proliferation of rheumatoid arthritis synovial fibroblasts and the production of COX‑2, PGE2 and MMPs
DC Field | Value | Language |
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dc.contributor.author | Yoon, Ha-Yong | - |
dc.contributor.author | Lee, Eun-Gyeong | - |
dc.contributor.author | Lee, Hyun | - |
dc.contributor.author | Cho, In Jin | - |
dc.contributor.author | Choi, Yun Jung | - |
dc.contributor.author | Sung, Myung-Soon | - |
dc.contributor.author | Yoo, Han-Gyul | - |
dc.contributor.author | Yoo, Wan-Hee | - |
dc.date.accessioned | 2022-07-16T07:50:15Z | - |
dc.date.available | 2022-07-16T07:50:15Z | - |
dc.date.created | 2021-05-13 | - |
dc.date.issued | 2013-10 | - |
dc.identifier.issn | 1107-3756 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/161708 | - |
dc.description.abstract | Inflammatory cytokines, matrix metalloproteinases (MMPs) and cyclooxygenase (COX)-2 released from rheumatoid arthritis synovial fibroblasts (RASFs) are involved in the destruction of both articular bone and cartilage. Kaempferol has been reported to act as an antioxidant and anti-inflammatory agent by inhibiting nitric oxide synthase and COX enzymes. The aim of the present study was to determine the effects of kaempferol on the interleukin-1 beta (IL-1 beta)-induced proliferation of RASFs and the production of MMPs, COX and prostaglandin E2 (PGE2) by RASFs. The proliferation of the RASFs stimulated with IL-1 beta and treated with/without kaempferol was evaluated by CCK-8 assay. The expression of MMPs, TIMP metallopeptidase inhibitor-1 (TIMP-1), COXs, PGE2 and that of intracellular MAPK signaling molecules, including p-ERK, p-p38, p-JNK and nuclear factor-kappa B (NF-kappa B) was examined by immunoblotting or semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and ELISA under the conditions described above. Kaempferol inhibited the proliferation of both unstimulated and IL-1 beta-stimulated RASFs, as well as the mRNA and protein expression of MMP-1, MMP-3, COX-2 and PGE2 induced by IL-1 beta. Kaempferol also inhibited the phosphorylation of ERK-1/2, p38 and JNK, as well as the activation of NF-kappa B induced by IL-1 beta. These results indicate that kaempferol inhibits synovial fibroblast proliferation, as well as the production of and MMPs, COX-2 and PGE2, which is involved in articular inflammation and destruction in rheumatoid arthritis (RA). Our data suggest that kaempferol may be a novel therapeutic agent for the treatment of RA. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SPANDIDOS PUBL LTD | - |
dc.title | Kaempferol inhibits IL‑1β‑induced proliferation of rheumatoid arthritis synovial fibroblasts and the production of COX‑2, PGE2 and MMPs | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Hyun | - |
dc.identifier.doi | 10.3892/ijmm.2013.1468 | - |
dc.identifier.scopusid | 2-s2.0-84883324461 | - |
dc.identifier.wosid | 000323725200030 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, v.32, no.4, pp.971 - 977 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | - |
dc.citation.title | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | - |
dc.citation.volume | 32 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 971 | - |
dc.citation.endPage | 977 | - |
dc.type.rims | ART | - |
dc.type.docType | 정기학술지(Article(Perspective Article포함)) | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.subject.keywordPlus | COLLAGEN-INDUCED ARTHRITIS | - |
dc.subject.keywordPlus | DOWN-REGULATION | - |
dc.subject.keywordPlus | JOINT INFLAMMATION | - |
dc.subject.keywordPlus | TNF-ALPHA | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | FLAVONOIDS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | DESTRUCTION | - |
dc.subject.keywordPlus | QUERCETIN | - |
dc.subject.keywordAuthor | cyclooxygenase | - |
dc.subject.keywordAuthor | interleukin-1 beta | - |
dc.subject.keywordAuthor | prostaglandin E2 | - |
dc.subject.keywordAuthor | matrix metalloproteinases | - |
dc.subject.keywordAuthor | rheumatoid arthritis | - |
dc.subject.keywordAuthor | kaempferol | - |
dc.identifier.url | https://www.spandidos-publications.com/10.3892/ijmm.2013.1468 | - |
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