Dexamethasone-conjugated polyethylenimine/MIF siRNA complex regulation of particulate matter-induced airway inflammation
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Moonhwan | - |
dc.contributor.author | Lee, Minhyung | - |
dc.contributor.author | Rhim, Taiyoun | - |
dc.date.accessioned | 2022-07-16T07:56:12Z | - |
dc.date.available | 2022-07-16T07:56:12Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2013-10 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/161795 | - |
dc.description.abstract | Inhalation of airborne particulate matter (PM), such as silicon dioxide (SiO2) and titanium dioxide (TiO2), induces acute lung inflammation. siRNA therapy has been proposed as a method to repair acute lung inflammation. To determine whether DEXA-PEI/MIF siRNA contributes to SiO2-induced acute lung inflammation repair, we administered Dexa-PEI/MIF siRNA in SiO2-treated Beas-2b cells and instilled DEXA-PEI-MIF siRNA intratracheally in mice with SiO2-induced acute lung inflammation. Using genetic (MIF mRNA RT-PCR), histological (H&E and PAS) and immunohistochemical (MIF and Muc5ac) analyses, we estimated the acute lung inflammation in Beas-2b cells and BALB/c mice. Cells and mice treated with SiO2 particles demonstrated pulmonary inflammation. DEXA-PEI/MIF siRNA restricted the extent of the pulmonary inflammation reaction to SiO2 in cells and mice. In case of SiO2-treated Beas-2b cells, only DEXA-PEI treatment failed to effectively regulate MIF mRNA release. At the same time, only DEXA-PEI treatment adjusted the amount of MIF mRNA to some extent in SiO2-treated BALB/c mice. siRNA treatment did not markedly control MIF mRNA release in mice. We also observed that the amount of MIF mRNA was decreased in cells and mice treated with DEXA-PEI/MIF siRNA. The increase of MIF mRNA markedly increased Muc5ac; in contrast, the decrease of MIF mRNA using DEXA-PEI/MIF siRNA effectively lowered Muc5ac in SiO2-treated cells and mice. These results suggest that DEXA-PEI plays a role in delivering siRNA to the nucleus as a carrier and limits the extent of acute lung inflammation. MIF siRNA also contributed to the reparative lung response in SiO2-induced pulmonary inflammation. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | Dexamethasone-conjugated polyethylenimine/MIF siRNA complex regulation of particulate matter-induced airway inflammation | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Minhyung | - |
dc.contributor.affiliatedAuthor | Rhim, Taiyoun | - |
dc.identifier.doi | 10.1016/j.biomaterials.2013.05.082 | - |
dc.identifier.scopusid | 2-s2.0-84880506897 | - |
dc.identifier.wosid | 000322931900025 | - |
dc.identifier.bibliographicCitation | BIOMATERIALS, v.34, no.30, pp.7453 - 7461 | - |
dc.relation.isPartOf | BIOMATERIALS | - |
dc.citation.title | BIOMATERIALS | - |
dc.citation.volume | 34 | - |
dc.citation.number | 30 | - |
dc.citation.startPage | 7453 | - |
dc.citation.endPage | 7461 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.subject.keywordPlus | MIGRATION-INHIBITORY FACTOR | - |
dc.subject.keywordPlus | GOBLET CELL HYPERPLASIA | - |
dc.subject.keywordPlus | FACTOR MIF | - |
dc.subject.keywordPlus | LUNG-FUNCTION | - |
dc.subject.keywordPlus | TIME-SERIES | - |
dc.subject.keywordPlus | POLLUTION | - |
dc.subject.keywordPlus | ASSOCIATION | - |
dc.subject.keywordPlus | ASTHMA | - |
dc.subject.keywordPlus | VITRO | - |
dc.subject.keywordPlus | HYPERSENSITIVITY | - |
dc.subject.keywordAuthor | PM (particulate matter) | - |
dc.subject.keywordAuthor | Airway inflammation | - |
dc.subject.keywordAuthor | DEXA-PEI | - |
dc.subject.keywordAuthor | MIF | - |
dc.subject.keywordAuthor | siRNA | - |
dc.subject.keywordAuthor | Gene delivery | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0142961213006741?via%3Dihub | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.