3D printed micro-chambers carrying stem cell spheroids and pro-proliferative growth factors for bone tissue regeneration
DC Field | Value | Language |
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dc.contributor.author | Lee, Jinkyu | - |
dc.contributor.author | Seok, Ji Min | - |
dc.contributor.author | Huh, Seung Jae | - |
dc.contributor.author | Byun, Hayeon | - |
dc.contributor.author | Lee, Sangmin | - |
dc.contributor.author | Park, Su A. | - |
dc.contributor.author | Shin, Heungsoo | - |
dc.date.accessioned | 2021-07-30T04:50:36Z | - |
dc.date.available | 2021-07-30T04:50:36Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2021-01 | - |
dc.identifier.issn | 1758-5082 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/1617 | - |
dc.description.abstract | Three-dimensional (3D)-printed scaffolds have proved to be effective tools for delivering growth factors and cells in bone-tissue engineering. However, delivering spheroids that enhance cellular function remains challenging because the spheroids tend to suffer from low viability, which limits bone regeneration in vivo. Here, we describe a 3D-printed polycaprolactone micro-chamber that can deliver human adipose-derived stem cell spheroids. An in vitro culture of cells from spheroids in the micro-chamber exhibited greater viability and proliferation compared with cells cultured without the chamber. We coated the surface of the chamber with 500 ng of platelet-derived growth factors (PDGFs), and immobilized 50 ng of bone morphogenetic protein 2 (BMP-2) on fragmented fibers, which were incorporated within the spheroids as a new platform for a dual-growth-factor delivery system. The PDGF detached from the chamber within 8 h and the remains were retained on the surface of chamber while the BMP-2 was entrapped by the spheroid. In vitro osteogenic differentiation of the cells from the spheroids in the micro-chamber with dual growth factors enhanced alkaline phosphatase and collagen type 1A expression by factors of 126.7 +/- 19.6 and 89.7 +/- 0.3, respectively, compared with expression in a micro-chamber with no growth factors. In vivo transplantation of the chambers with dual growth factors into mouse calvarial defects resulted in a 77.0 +/- 15.9% of regenerated bone area, while the chamber without growth factors and a defect-only group achieved 7.6 +/- 3.9% and 5.0 +/- 1.9% of regenerated bone areas, respectively. These findings indicate that a spheroid-loaded micro-chamber supplied with dual growth factors can serve as an effective protein-delivery platform that increases stem-cell functioning and bone regeneration. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | IOP PUBLISHING LTD | - |
dc.title | 3D printed micro-chambers carrying stem cell spheroids and pro-proliferative growth factors for bone tissue regeneration | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Shin, Heungsoo | - |
dc.identifier.doi | 10.1088/1758-5090/abc39c | - |
dc.identifier.scopusid | 2-s2.0-85099110698 | - |
dc.identifier.wosid | 000599793200001 | - |
dc.identifier.bibliographicCitation | BIOFABRICATION, v.13, no.1, pp.1 - 19 | - |
dc.relation.isPartOf | BIOFABRICATION | - |
dc.citation.title | BIOFABRICATION | - |
dc.citation.volume | 13 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 19 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.subject.keywordPlus | OSTEOGENIC DIFFERENTIATION | - |
dc.subject.keywordPlus | SCAFFOLDS | - |
dc.subject.keywordPlus | POLYDOPAMINE | - |
dc.subject.keywordPlus | LIMITATIONS | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | RHBMP-2 | - |
dc.subject.keywordPlus | BB | - |
dc.subject.keywordAuthor | 3D printing | - |
dc.subject.keywordAuthor | dual growth factor delivery | - |
dc.subject.keywordAuthor | osteoinductive spheroid | - |
dc.subject.keywordAuthor | bone regeneration | - |
dc.subject.keywordAuthor | micro-chamber | - |
dc.identifier.url | https://iopscience.iop.org/article/10.1088/1758-5090/abc39c | - |
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