The phenyl-thiophenyl propenone RK-I-123 reduces the levels of reactive oxygen species and suppresses the activation of NF-kappa B and AP-1 and IL-8 expression in Helicobacter pylori-infected gastric epithelial AGS cells
DC Field | Value | Language |
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dc.contributor.author | Jang, Sung Hee | - |
dc.contributor.author | Cho, Soonok | - |
dc.contributor.author | Lee, Eung-Seok | - |
dc.contributor.author | Kim, Jung Mogg | - |
dc.contributor.author | Kim, Hyeyoung | - |
dc.date.accessioned | 2022-07-16T09:12:23Z | - |
dc.date.available | 2022-07-16T09:12:23Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2013-07 | - |
dc.identifier.issn | 1023-3830 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/162401 | - |
dc.description.abstract | To investigate whether the phenyl-thiophenyl propenone RK-I-123 suppresses interleukin-8 (IL-8) expression and activation of mitogen-activated protein kinases (MAPKs) and transcription factors (nuclear factor-kappa B [NF-kappa B] and activator protein-1 [AP-1]) by reducing reactive oxygen species (ROS) levels in Helicobacter pylori-infected gastric epithelial cells. Helicobacter pylori in Korean isolates, human gastric epithelial AGS cells. AGS cells pretreated with or without RK-I-123 were cultured in the presence of H. pylori at a bacterium/cell ratio of 300:1. Reactive oxygen species and IL-8 levels were determined by dichlorofluorescein fluorescence and enzyme-linked immunosorbent assay. The IL-8 mRNA expression was analyzed by the real-time reverse transcription-polymerase chain reaction (RT-PCR). The MAPK and I kappa B alpha levels were determined by western blotting. The activation of NF-kappa B and AP-1 was determined by the electrophoretic mobility shift assay. Helicobacter pylori induced an increase in ROS and IL-8 expression and activation of MAPKs and transcription factors (NF-kappa B and AP-1) together with the degradation of I kappa B alpha in AGS cells, all of which were inhibited by RK-I-123. The RK-I-123 suppressed the H. pylori-induced IL-8 expression and activation of MAPKs, NF-kappa B, and AP-1 by reducing ROS levels in AGS cells. The RK-I-123 may be a potential candidate for the treatment of H. pylori-induced gastric inflammation. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER BASEL AG | - |
dc.title | The phenyl-thiophenyl propenone RK-I-123 reduces the levels of reactive oxygen species and suppresses the activation of NF-kappa B and AP-1 and IL-8 expression in Helicobacter pylori-infected gastric epithelial AGS cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Jung Mogg | - |
dc.identifier.doi | 10.1007/s00011-013-0621-4 | - |
dc.identifier.scopusid | 2-s2.0-84879204390 | - |
dc.identifier.wosid | 000320377500007 | - |
dc.identifier.bibliographicCitation | INFLAMMATION RESEARCH, v.62, no.7, pp.689 - 696 | - |
dc.relation.isPartOf | INFLAMMATION RESEARCH | - |
dc.citation.title | INFLAMMATION RESEARCH | - |
dc.citation.volume | 62 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 689 | - |
dc.citation.endPage | 696 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.subject.keywordPlus | NITRIC-OXIDE SYNTHASE | - |
dc.subject.keywordPlus | INTERLEUKIN-8 PRODUCTION | - |
dc.subject.keywordPlus | INHIBITORY-ACTIVITIES | - |
dc.subject.keywordPlus | NADPH OXIDASE | - |
dc.subject.keywordPlus | CYCLOOXYGENASE-2 | - |
dc.subject.keywordPlus | 5-LIPOXYGENASE | - |
dc.subject.keywordPlus | STIMULATION | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | KINASES | - |
dc.subject.keywordPlus | MUCOSA | - |
dc.subject.keywordAuthor | Helicobacter pylori | - |
dc.subject.keywordAuthor | RK-I-123 | - |
dc.subject.keywordAuthor | IL-8 | - |
dc.subject.keywordAuthor | NF-kappa B | - |
dc.subject.keywordAuthor | Gastric epithelial cells | - |
dc.identifier.url | https://link.springer.com/article/10.1007%2Fs00011-013-0621-4 | - |
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