20-O-β-d-glucopyranosyl-20(S)-protopanaxadiol, a metabolite of ginseng, inhibits colon cancer growth by targeting TRPC channel-mediated calcium influx.
DC Field | Value | Language |
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dc.contributor.author | Hwang, Jeong Ah | - |
dc.contributor.author | Hwang, Mun Kyung | - |
dc.contributor.author | Jang, Yong woo | - |
dc.contributor.author | Lee, Eun Jung | - |
dc.contributor.author | Kim, Jong-Eun | - |
dc.contributor.author | Oh, Mi Hyun | - |
dc.contributor.author | Shin, Dong Joo | - |
dc.contributor.author | Lim, Semi | - |
dc.contributor.author | Ji, Geun Og | - |
dc.contributor.author | Oh, Uhtaek | - |
dc.contributor.author | Bode, Ann M. | - |
dc.contributor.author | Dong, Zigang | - |
dc.contributor.author | Lee, Ki Won | - |
dc.contributor.author | Lee, Hyong Joo | - |
dc.date.accessioned | 2022-07-16T09:29:40Z | - |
dc.date.available | 2022-07-16T09:29:40Z | - |
dc.date.created | 2021-05-13 | - |
dc.date.issued | 2013-06 | - |
dc.identifier.issn | 0955-2863 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/162525 | - |
dc.description.abstract | Abnormal regulation of Ca2+ mediates tumorigenesis and Ca2+ channels are reportedly deregulated in cancers, indicating that regulating Ca2+ signaling in cancer cells is considered as a promising strategy to treat cancer. However, little is known regarding the mechanism by which Ca2+ affects cancer cell death. Here, we show that 20-O-beta-D-glucopyranosyl-20(S)-protopanaxadiol (20-GPPD), a metabolite of ginseng saponin, causes apoptosis of colon cancer cells through the induction of cytoplasmic Ca2+center dot 20-GPPD decreased cell viability, increased annexin V-positive early apoptosis and induced sub-G1 accumulation and nuclear condensation of CT-26 murine colon cancer cells. Although 20-GPPD-induced activation of AMP-activated protein kinase (AMPK) played a key role in the apoptotic death of CT-26 cells, LKB1, a well-known upstream kinase of AMPK, was not involved in this activation. To identify the upstream target of 20-GPPD for activating AMPK, we examined the effect of Ca2+ on apoptosis of CT-26 cells. A calcium chelator recovered 20-GPPD-induced AMPK phosphorylation and CT-26 cell death. Confocal microscopy showed that 20-GPPD increased Ca2+ entry into CT-26 cells, whereas a transient receptor potential canonical (TRPC) blocker suppressed Ca2+ entry. When cells were treated with a TRPC blocker plus an endoplasmic reticulum (ER) calcium blocker, 20-GPPD-induced calcium influx was completely inhibited, suggesting that the ER calcium store, as well as TRPC, was involved. In vivo mouse CT-26 allografts showed that 20-GPPD significantly suppressed tumor growth, volume and weight in a dose-dependent manner. Collectively, 20-GPPD exerts potent anticarcinogenic effects on colon carcinogenesis by increasing Ca2+ influx, mainly through TRPC channels, and by targeting AMPK. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE INC | - |
dc.title | 20-O-β-d-glucopyranosyl-20(S)-protopanaxadiol, a metabolite of ginseng, inhibits colon cancer growth by targeting TRPC channel-mediated calcium influx. | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Jang, Yong woo | - |
dc.identifier.doi | 10.1016/j.jnutbio.2012.08.008 | - |
dc.identifier.scopusid | 2-s2.0-84878020374 | - |
dc.identifier.wosid | 000320096400020 | - |
dc.identifier.bibliographicCitation | JOURNAL OF NUTRITIONAL BIOCHEMISTRY, v.24, no.6, pp.1096 - 1104 | - |
dc.relation.isPartOf | JOURNAL OF NUTRITIONAL BIOCHEMISTRY | - |
dc.citation.title | JOURNAL OF NUTRITIONAL BIOCHEMISTRY | - |
dc.citation.volume | 24 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 1096 | - |
dc.citation.endPage | 1104 | - |
dc.type.rims | ART | - |
dc.type.docType | 정기학술지(Article(Perspective Article포함)) | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Nutrition & Dietetics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Nutrition & Dietetics | - |
dc.subject.keywordPlus | ACTIVATED PROTEIN-KINASE | - |
dc.subject.keywordPlus | KAPPA-B ACTIVATION | - |
dc.subject.keywordPlus | COMPOUND-K | - |
dc.subject.keywordPlus | INTESTINAL METABOLITE | - |
dc.subject.keywordPlus | SIGNALING PATHWAYS | - |
dc.subject.keywordPlus | UPSTREAM KINASE | - |
dc.subject.keywordPlus | CELL-DEATH | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | SAPONIN | - |
dc.subject.keywordAuthor | Calcium influx | - |
dc.subject.keywordAuthor | Colon cancer | - |
dc.subject.keywordAuthor | 20-GPPD | - |
dc.subject.keywordAuthor | TRPC | - |
dc.identifier.url | https://linkinghub.elsevier.com/retrieve/pii/S0955286312002252 | - |
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