Conversion of levulinic acid to 2-butanone by acetoacetate decarboxylase from Clostridium acetobutylicum
DC Field | Value | Language |
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dc.contributor.author | Min, Kyoungseon | - |
dc.contributor.author | Kim, Seil | - |
dc.contributor.author | Yum, Taewoo | - |
dc.contributor.author | Kim, Yunje | - |
dc.contributor.author | Sang, Byoung-In | - |
dc.contributor.author | Um, Youngsoon | - |
dc.date.accessioned | 2022-07-16T09:39:07Z | - |
dc.date.available | 2022-07-16T09:39:07Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2013-06 | - |
dc.identifier.issn | 0175-7598 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/162642 | - |
dc.description.abstract | In this study, a novel system for synthesis of 2-butanone from levulinic acid (gamma-keto-acid) via an enzymatic reaction was developed. Acetoacetate decarboxylase (AADC; E.C. 4.1.1.4) from Clostridium acetobutylicum was selected as a biocatalyst for decarboxylation of levulinic acid. The purified recombinant AADC from Escherichia coli successfully converted levulinic acid to 2-butanone with a conversion yield of 8.4-90.3 % depending on the amount of AADC under optimum conditions (30 A degrees C and pH 5.0) despite that acetoacetate, a beta-keto-acid, is a natural substrate of AADC. In order to improve the catalytic efficiency, an AADC-mediator system was tested using methyl viologen, methylene blue, azure B, zinc ion, and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) as mediators. Among them, methyl viologen showed the best performance, increasing the conversion yield up to 6.7-fold in comparison to that without methyl viologen. The results in this study are significant in the development of a renewable method for the synthesis of 2-butanone from biomass-derived chemical, levulinic acid, through enzymatic decarboxylation. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER | - |
dc.title | Conversion of levulinic acid to 2-butanone by acetoacetate decarboxylase from Clostridium acetobutylicum | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Sang, Byoung-In | - |
dc.identifier.doi | 10.1007/s00253-013-4879-9 | - |
dc.identifier.scopusid | 2-s2.0-84878679101 | - |
dc.identifier.wosid | 000319609400041 | - |
dc.identifier.bibliographicCitation | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.97, no.12, pp.5627 - 5634 | - |
dc.relation.isPartOf | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.title | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 97 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 5627 | - |
dc.citation.endPage | 5634 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | OXIDATIVE DECARBOXYLATION | - |
dc.subject.keywordPlus | TRANSPORTATION FUELS | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | LACCASE | - |
dc.subject.keywordPlus | BUTANOL | - |
dc.subject.keywordPlus | DECOLORIZATION | - |
dc.subject.keywordPlus | MEDIATORS | - |
dc.subject.keywordPlus | BIOFUELS | - |
dc.subject.keywordPlus | STRAW | - |
dc.subject.keywordAuthor | Enzymatic decarboxylation | - |
dc.subject.keywordAuthor | Acetoacetate decarboxylase | - |
dc.subject.keywordAuthor | Levulinic acid | - |
dc.subject.keywordAuthor | 2-Butanone | - |
dc.identifier.url | https://link.springer.com/article/10.1007%2Fs00253-013-4879-9 | - |
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