Silaffin Peptides as a Novel Signal Enhancer for Gravimetric Biosensors
DC Field | Value | Language |
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dc.contributor.author | Dong Hyun Nam | - |
dc.contributor.author | Lee, Jeong-O | - |
dc.contributor.author | Sang, Byoung-In | - |
dc.contributor.author | Won, Keehoon | - |
dc.contributor.author | Kim, Yong Hwan | - |
dc.date.accessioned | 2022-07-16T10:09:02Z | - |
dc.date.available | 2022-07-16T10:09:02Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2013-05 | - |
dc.identifier.issn | 0273-2289 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/162867 | - |
dc.description.abstract | Application of biomimetic silica formation to gravimetric biosensors has been conducted for the first time. As a model system, silaffin peptides fused with green fluorescent protein (GFP) were immobilized on a gold quartz crystal resonator for quartz crystal microbalances using a self-assembled monolayer. When a solution of silicic acid was supplied, silica particles were successfully deposited on the Au surface, resulting in a significant change in resonance frequency (i.e., signal enhancement) with the silaffin-GFP. However, frequency was not altered when bare GFP was used as a control. The novel peptide enhancer is advantageous because it can be readily and quantitatively conjugated with sensing proteins using recombinant DNA technology. As a proof of concept, this study shows that the silaffin domains can be employed as a novel and efficient biomolecular signal enhancer for gravimetric biosensors. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | HUMANA PRESS INC | - |
dc.title | Silaffin Peptides as a Novel Signal Enhancer for Gravimetric Biosensors | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Sang, Byoung-In | - |
dc.identifier.doi | 10.1007/s12010-013-0161-y | - |
dc.identifier.scopusid | 2-s2.0-84876682983 | - |
dc.identifier.wosid | 000317923600003 | - |
dc.identifier.bibliographicCitation | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, v.170, no.1, pp.25 - 31 | - |
dc.relation.isPartOf | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | - |
dc.citation.title | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | - |
dc.citation.volume | 170 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 25 | - |
dc.citation.endPage | 31 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | QUARTZ-CRYSTAL MICROBALANCE | - |
dc.subject.keywordPlus | ENZYME IMMOBILIZATION | - |
dc.subject.keywordPlus | CHIMERIC PROTEINS | - |
dc.subject.keywordPlus | SILICA | - |
dc.subject.keywordPlus | ENTRAPMENT | - |
dc.subject.keywordPlus | BIOSILICA | - |
dc.subject.keywordPlus | SUPPORT | - |
dc.subject.keywordAuthor | Silaffin peptides | - |
dc.subject.keywordAuthor | Biomimetic silica formation | - |
dc.subject.keywordAuthor | R5 peptide | - |
dc.subject.keywordAuthor | Quartz crystal microbalances | - |
dc.subject.keywordAuthor | Signal enhancer | - |
dc.identifier.url | https://link.springer.com/article/10.1007%2Fs12010-013-0161-y | - |
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