Establishing an in vivo p48ZnF bioluminescence mouse brain imaging model

Abstract

p48ZnF is a C3H1 zinc finger domain-containing protein that is involved in the control of gene transcription and translation. In the present study a novel transgenic p48ZnF mouse model is described that is useful for in vivo brain imaging using luciferase as bioluminescence-mediating reporter gene. Yeast two-hybrid screening and western blot analyses revealed Drg1 (developmentally regulated GTP binding protein 1) and Pcbp1 (poly (rC)-binding protein 1) as p48ZnF-associated proteins. Interestingly, p48ZnF' cellular location of action depends on the cell's differentiation status: nuclear in proliferating cells and cytoplasmic in differentiated neurons.