Autophagy regulates chlorpyrifos-induced apoptosis in SH-SY5Y cells
DC Field | Value | Language |
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dc.contributor.author | Park, Jae Hyeon | - |
dc.contributor.author | Lee, Jeong Eun | - |
dc.contributor.author | Shin, In Chul | - |
dc.contributor.author | Koh, Hyun Chul | - |
dc.date.accessioned | 2022-07-16T10:27:13Z | - |
dc.date.available | 2022-07-16T10:27:13Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2013-04 | - |
dc.identifier.issn | 0041-008X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/163072 | - |
dc.description.abstract | Recent studies have shown that up-regulation of autophagy may be a tractable therapeutic intervention for clearing disease-causing proteins, including alpha-synuclein, ubiquitin, and other misfolded or aggregated proteins in pesticide-induced neurodegeneration. In a previous study, we reported that chlorpyrifos (CPF)-induced mitochondria-dependent apoptosis is mediated through reactive oxygen species in SH-SY5Y cells. In this study, we explored a novel pharmacotherapeutic approach to prevent CPF neurotoxicity involving the regulation of autophagy. We investigated the modulation of CPF-induced apoptosis according to autophagy regulation. We found that CPF induced apoptosis in SH-SY5Y cells, as demonstrated by the activation of caspase-3 and nuclear condensation. In addition, we observed that cells treated with CPF underwent autophagic cell death by monitoring the expression of LC3-II and p62. Pretreatment with the autophagy inducer rapamycin significantly enhanced the cell viability of CPF-exposed cells, and the enhancement of cell viability was partially due to alleviation of CPF- induced apoptosis via a decrease in levels of cleaved caspase-3. Specifically, rapamycin pretreatment decreased Bax and increased Bcl-2 expression in mitochondria. In addition, rapamycin significantly decreased cytochrome c release in from mitochondria into the cytosol. However, pretreatment of cells with the autophagy inhibitor, 3-methyladenine (3MA), remarkably increased CPF toxicity in these cells; this with correlated with increased expression of Box and decreased expression of Bcl-2 in mitochondria. Our results suggest that CPF-induced cytotoxicity is modified by autophagy regulation and that rapamycin protects against CPF-induced apoptosis by enhancing autophagy. Pharmacologic induction of autophagy by rapamycin may be a useful treatment strategy in neurodegenerative disorders. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | Autophagy regulates chlorpyrifos-induced apoptosis in SH-SY5Y cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Shin, In Chul | - |
dc.contributor.affiliatedAuthor | Koh, Hyun Chul | - |
dc.identifier.doi | 10.1016/j.taap.2013.01.013 | - |
dc.identifier.scopusid | 2-s2.0-84873889399 | - |
dc.identifier.wosid | 000316305600006 | - |
dc.identifier.bibliographicCitation | TOXICOLOGY AND APPLIED PHARMACOLOGY, v.268, no.1, pp.55 - 67 | - |
dc.relation.isPartOf | TOXICOLOGY AND APPLIED PHARMACOLOGY | - |
dc.citation.title | TOXICOLOGY AND APPLIED PHARMACOLOGY | - |
dc.citation.volume | 268 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 55 | - |
dc.citation.endPage | 67 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalResearchArea | Toxicology | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Toxicology | - |
dc.subject.keywordPlus | PARKINSONS-DISEASE | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | NEURODEGENERATIVE DISORDERS | - |
dc.subject.keywordPlus | BAX TRANSLOCATION | - |
dc.subject.keywordPlus | NEURON DEATH | - |
dc.subject.keywordPlus | MAP KINASES | - |
dc.subject.keywordPlus | LIFE-SPAN | - |
dc.subject.keywordPlus | RAPAMYCIN | - |
dc.subject.keywordPlus | MITOCHONDRIA | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordAuthor | Chlorpyrifos | - |
dc.subject.keywordAuthor | Autophagy | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | Neuroprotection | - |
dc.subject.keywordAuthor | Rapamycin | - |
dc.subject.keywordAuthor | SH-SY5Y cells | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0041008X13000343?via%3Dihub | - |
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