Improved transplantation outcome through delivery of DNA encoding secretion signal peptide-linked glucagon-like peptide-1 into mouse islets
DC Field | Value | Language |
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dc.contributor.author | Chae, Hee Young | - |
dc.contributor.author | Lee, Minhyung | - |
dc.contributor.author | Hwang, Hyo Jeong | - |
dc.contributor.author | Kim, Hyun Ah | - |
dc.contributor.author | Kang, Jun Goo | - |
dc.contributor.author | Kim, Chul Sik | - |
dc.contributor.author | Lee, Seong Jin | - |
dc.contributor.author | Ihm, Sung-Hee | - |
dc.date.accessioned | 2022-07-16T10:27:38Z | - |
dc.date.available | 2022-07-16T10:27:38Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2013-04 | - |
dc.identifier.issn | 0934-0874 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/163076 | - |
dc.description.abstract | Glucagon-like peptide-1 (GLP-1) stimulates cell proliferation and has anti-apoptotic effects on pancreatic islet cells. In our previous study, the transduction of mouse islets with a recombinant adenovirus containing GLP-1 cDNA enhanced islet graft survival. In this study, we sought to deliver the GLP-1 gene using a nonviral vector, which raises fewer safety issues in clinical application. We constructed a plasmid, p-SP-GLP-1, in which a secretion signal peptide (SP) was inserted to increase GLP-1 secretion, and transfected mouse islets using the nonviral carrier Effectene. Transfection of p-SP-GLP-1 induced a significant increase in bioactive GLP-1 levels in islet cultures. Islets transfected with p-SP-GLP-1 were protected from H2O2-induced cell damage in vitro. In addition, glucose-stimulated insulin secretion was significantly increased in p-SP-GLP-1-transfected islets. Diabetic syngeneic mice transplanted under the kidney capsule with a marginal mass of p-SP-GLP-1-transfected islets rapidly became normoglycemic, with 88% of recipients being normoglycemic at 30days post-transplantation compared with 52% of mice that received p-transfected islet grafts (P<0.05). Islet grafts retrieved 7days after transplantation revealed that the p-SP-GLP-1-transfected group had significantly more Ki67-positive cells as compared with the p-transfected group. In conclusion, delivery of a plasmid containing a secretion SP and GLP-1 cDNA using a nonviral carrier leads to efficient secretion of GLP-1 in mouse islet cells, enhances islet cell survival during the early post-transplant period, and improves islet transplantation outcome. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.title | Improved transplantation outcome through delivery of DNA encoding secretion signal peptide-linked glucagon-like peptide-1 into mouse islets | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Minhyung | - |
dc.identifier.doi | 10.1111/tri.12052 | - |
dc.identifier.scopusid | 2-s2.0-84875382430 | - |
dc.identifier.wosid | 000316633100017 | - |
dc.identifier.bibliographicCitation | TRANSPLANT INTERNATIONAL, v.26, no.4, pp.443 - 452 | - |
dc.relation.isPartOf | TRANSPLANT INTERNATIONAL | - |
dc.citation.title | TRANSPLANT INTERNATIONAL | - |
dc.citation.volume | 26 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 443 | - |
dc.citation.endPage | 452 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Surgery | - |
dc.relation.journalResearchArea | Transplantation | - |
dc.relation.journalWebOfScienceCategory | Surgery | - |
dc.relation.journalWebOfScienceCategory | Transplantation | - |
dc.subject.keywordPlus | GROWTH-FACTOR GENE | - |
dc.subject.keywordPlus | HUMAN PANCREATIC-ISLETS | - |
dc.subject.keywordPlus | BETA-CELL REPLICATION | - |
dc.subject.keywordPlus | DIABETIC-RATS | - |
dc.subject.keywordPlus | FOLLOW-UP | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | EXENDIN-4 | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | GLP-1 | - |
dc.subject.keywordPlus | MASS | - |
dc.subject.keywordAuthor | glucagon-like peptide-1 | - |
dc.subject.keywordAuthor | islet | - |
dc.subject.keywordAuthor | transplantation | - |
dc.identifier.url | https://onlinelibrary.wiley.com/doi/10.1111/tri.12052 | - |
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