Inhibitory effects of eupatilin on tumor invasion of human gastric cancer MKN-1 cells
DC Field | Value | Language |
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dc.contributor.author | Park, Byeong Bae | - |
dc.contributor.author | Yoon, Jin Sun | - |
dc.contributor.author | Kim, Eun Shil | - |
dc.contributor.author | Choi, Jiyoung | - |
dc.contributor.author | Won, Young Woong | - |
dc.contributor.author | Choi, Jung Hye | - |
dc.contributor.author | Lee, Young Yiul | - |
dc.date.accessioned | 2022-07-16T10:39:15Z | - |
dc.date.available | 2022-07-16T10:39:15Z | - |
dc.date.created | 2021-05-11 | - |
dc.date.issued | 2013-04 | - |
dc.identifier.issn | 1010-4283 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/163132 | - |
dc.description.abstract | Extracts of the whole herb of Artemisia asiatica Nakai (Asteraceae) are used in traditional oriental medicine to treat inflammation. Eupatilin (5,7-dihydroxy-3',4',6-trimethoxyflavone) is one of the pharmacologically active components found in A. asiatica, and has been shown to possess anti-tumoral effects in some malignancies, including gastric cancer. However, its anti-metastatic effect in gastric cancer is hardly known. In this study, anti-metastatic effect of eupatilin was investigated in the human gastric cancer cell line, MKN-1. Eupatilin inhibited MKN-1 growth in a dose- and a time-dependent manner, and induced apoptosis with a concomitant increase of caspase-3 activity. ELISA demonstrated that release of pro-inflammatory cytokines (IL-1 beta, TNF-alpha, IL-6, and IL-8) was significantly reduced by eupatilin. And p-AKT and p-ERK (p44/42) was reduced. Expression level of beta-catenin and integrin was reduced and p-GSK beta was increased. In transcription reporter system, the activity of the transcriptional factor, NF-kappa B, was reduced by eupatilin and the expression of p65 was down-regulated when MKN-1 cells were treated with eupatilin. Moreover, a zymography study revealed that this reduction in invasive potential resulted from a reduction in type IV collagenolytic (gelatinolytic) activity. The expressions of metalloproteinases (MMP-2 and MMP-9) were also reduced in MKN-1 cells treated with eupatilin. In vitro invasion assay, eupatilin inhibited MKN-1 penetrating reconstituted basement membrane barriers. These results suggest that eupatilin inhibits the MKN-1 gastric cancer cell proliferation via activation of caspase-3 and the metastatic potential of gastric cancer cells via down-regulation of NF-kappa B activity followed by reduction of pro-inflammatory cytokine-mediated MMPs expressions. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SAGE PUBLICATIONS LTD | - |
dc.title | Inhibitory effects of eupatilin on tumor invasion of human gastric cancer MKN-1 cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Byeong Bae | - |
dc.contributor.affiliatedAuthor | Won, Young Woong | - |
dc.contributor.affiliatedAuthor | Choi, Jung Hye | - |
dc.identifier.doi | 10.1007/s13277-012-0621-y | - |
dc.identifier.scopusid | 2-s2.0-84877152685 | - |
dc.identifier.wosid | 000316364500032 | - |
dc.identifier.bibliographicCitation | TUMOR BIOLOGY, v.34, no.2, pp.875 - 885 | - |
dc.relation.isPartOf | TUMOR BIOLOGY | - |
dc.citation.title | TUMOR BIOLOGY | - |
dc.citation.volume | 34 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 875 | - |
dc.citation.endPage | 885 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.subject.keywordPlus | NECROSIS-FACTOR-ALPHA | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | HELICOBACTER-PYLORI INFECTION | - |
dc.subject.keywordPlus | BETA-CATENIN | - |
dc.subject.keywordPlus | EPITHELIAL-CELLS | - |
dc.subject.keywordPlus | UROKINASE RECEPTOR | - |
dc.subject.keywordPlus | ARTEMISIA PLANTS | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | UP-REGULATION | - |
dc.subject.keywordPlus | AGS CELLS | - |
dc.subject.keywordAuthor | Growth inhibition | - |
dc.subject.keywordAuthor | Gastric cancer | - |
dc.subject.keywordAuthor | Eupatilin | - |
dc.subject.keywordAuthor | Metastasis | - |
dc.subject.keywordAuthor | Invasion | - |
dc.identifier.url | https://link.springer.com/article/10.1007%2Fs13277-012-0621-y | - |
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