Optimal Formation of Genetically Modified and Functional Pancreatic Islet Spheroids by Using Hanging-Drop Strategy
DC Field | Value | Language |
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dc.contributor.author | Kim, Ha Jun | - |
dc.contributor.author | Alam, Zahid | - |
dc.contributor.author | Hwang, Jin Wook | - |
dc.contributor.author | Hwang, Yong Hwa | - |
dc.contributor.author | Kim, Mu-joong | - |
dc.contributor.author | Yoon, Sangtae | - |
dc.contributor.author | Byun, Youngro | - |
dc.contributor.author | Lee, Du-Yeong | - |
dc.date.accessioned | 2022-07-16T10:59:56Z | - |
dc.date.available | 2022-07-16T10:59:56Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2013-03 | - |
dc.identifier.issn | 0041-1345 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/163267 | - |
dc.description.abstract | Background. Rejection and hypoxia are important factors causing islet loss at an early stage after pancreatic islet transplantation. Recently, islets have been dissociated into single cells for reaggregation into so-called islet spheroids. Herein, we used a hanging-drop strategy to form islet spheroids to achieve functional equivalence to intact islets. Methods. To obtain single islet cells, we dissociated islets with trypsin-EDTA digestion for 10 minutes. To obtain spheroids, we dropped various numbers of single cells (125, 250, or 500 cells/30 mu L drop) onto a Petri dish, that was inverted for incubation in humidified air containing 5% CO2 at 37 degrees C for 7 days. The aggregated spheroids in the droplets were harvested for further culture. Results. The size of the aggregated islet spheroids depended on the number of single cells (125-500 cells/30 mu L droplet). Their morphology was similar to that of intact islets without any cellular damage. When treated with various concentrations of glucose to evaluate responsiveness, their glucose-mediated stimulation index value was similar to that of intact islets, an observation that was attributed to strong cell-to-cell interactions in islet spheroids. However, islet spheroids aggregated in general culture dishes showed abnormal glucose responsiveness owing to weak cell-to-cell interactions. Cell-to-cell interactions in islet spheroids were confirmed with an anti-connexin-36 monoclonal antibody. Finally, nonviral poly(ethylene imine) mediated interleukin-10 cytokine gene delivered beforehand into dissociated single cells before formation of islet spheroids increased the gene transfection efficacy and interleukin-10 secretion from islet spheroids >4-fold compared with intact islets. Conclusion. These results demonstrated the potential application of genetically modified, functional islet spheroids with of controlled size and morphology using an hanging-drop technique. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE INC | - |
dc.title | Optimal Formation of Genetically Modified and Functional Pancreatic Islet Spheroids by Using Hanging-Drop Strategy | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Du-Yeong | - |
dc.identifier.doi | 10.1016/j.transproceed.2012.11.014 | - |
dc.identifier.scopusid | 2-s2.0-84875139345 | - |
dc.identifier.wosid | 000316772500024 | - |
dc.identifier.bibliographicCitation | TRANSPLANTATION PROCEEDINGS, v.45, no.2, pp.605 - 610 | - |
dc.relation.isPartOf | TRANSPLANTATION PROCEEDINGS | - |
dc.citation.title | TRANSPLANTATION PROCEEDINGS | - |
dc.citation.volume | 45 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 605 | - |
dc.citation.endPage | 610 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalResearchArea | Surgery | - |
dc.relation.journalResearchArea | Transplantation | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.relation.journalWebOfScienceCategory | Surgery | - |
dc.relation.journalWebOfScienceCategory | Transplantation | - |
dc.subject.keywordPlus | GENE DELIVERY | - |
dc.subject.keywordPlus | ADENOASSOCIATED VIRUS | - |
dc.subject.keywordPlus | CELL COMMUNICATION | - |
dc.subject.keywordPlus | INSULIN-SECRETION | - |
dc.subject.keywordPlus | TRANSPLANTATION | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | TRANSDUCTION | - |
dc.subject.keywordPlus | VECTOR | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0041134512013504?via%3Dihub | - |
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