Cdk1 Protein-mediated Phosphorylation of Receptor-associated Protein 80 (RAP80) Serine 677 Modulates DNA Damage-induced G(2)/M Checkpoint and Cell Survival
DC Field | Value | Language |
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dc.contributor.author | Cho, Hyun Jung | - |
dc.contributor.author | Oh, Yun Jung | - |
dc.contributor.author | Han, Seung Hun | - |
dc.contributor.author | Chung, Hee Jin | - |
dc.contributor.author | Kim, Chang Hee | - |
dc.contributor.author | Lee, Nam Soo | - |
dc.contributor.author | Kim, Won-Ju | - |
dc.contributor.author | Choi, Je-Min | - |
dc.contributor.author | Kim, Hongtae | - |
dc.date.accessioned | 2022-07-16T11:22:10Z | - |
dc.date.available | 2022-07-16T11:22:10Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2013-02 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/163520 | - |
dc.description.abstract | Post-translational phosphorylation plays critical roles in the assembly of signaling and repair proteins in the DNA damage response pathway. RAP80, a component of the BRCA1-A complex, is crucial in cell cycle checkpoint activation and DNA damage repair. However, its molecular mechanism is unclear. In this study, we identified Cdk1 as a new RAP80-binding protein and demonstrated that the Cdk1-cyclin B-1 complex phosphorylates RAP80 at Ser-677 using an in vitro kinase assay and a phosphopeptide-specific antibody against phospho-Ser-677 of RAP80. RAP80 Ser-677 phosphorylation occurred in the M phase of the cell cycle when Cdk1 was in an active state. In addition, ionizing radiation (IR) induced RAP80 phosphorylation at Ser-677. Mutation of Ser-677 to alanine sensitized cells to IR and functioned in G(2)/M checkpoint control. These results suggest that post-translational phosphorylation of RAP80 by the Cdk1-cyclin B-1 complex is important for RAP80 functional sensitivity to IR and G(2)/M checkpoint control. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | - |
dc.title | Cdk1 Protein-mediated Phosphorylation of Receptor-associated Protein 80 (RAP80) Serine 677 Modulates DNA Damage-induced G(2)/M Checkpoint and Cell Survival | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Choi, Je-Min | - |
dc.identifier.doi | 10.1074/jbc.M112.401299 | - |
dc.identifier.scopusid | 2-s2.0-84873658685 | - |
dc.identifier.wosid | 000314845000008 | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.288, no.6, pp.3768 - 3776 | - |
dc.relation.isPartOf | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.title | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.volume | 288 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 3768 | - |
dc.citation.endPage | 3776 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | TARGETS BRCA1 | - |
dc.subject.keywordPlus | REPAIR | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | SITES | - |
dc.subject.keywordPlus | CYCLE | - |
dc.subject.keywordPlus | CANCERS | - |
dc.subject.keywordPlus | CCDC98 | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0021925820463379?via%3Dihub | - |
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