Epigenetic reprogramming in somatic cells induced by extract from germinal vesicle stage pig oocytes
DC Field | Value | Language |
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dc.contributor.author | Bui, Hong-Thuy | - |
dc.contributor.author | Kwon, Deug-Nam | - |
dc.contributor.author | Kang, Min-Hui | - |
dc.contributor.author | Oh, Mi-Hye | - |
dc.contributor.author | Park, Mi-Ryung | - |
dc.contributor.author | Park, Woo-Jin | - |
dc.contributor.author | Paik, Seung-Sam | - |
dc.contributor.author | Nguyen Van Thuan | - |
dc.contributor.author | Kim, Jin-Hoi | - |
dc.date.accessioned | 2022-07-16T12:31:43Z | - |
dc.date.available | 2022-07-16T12:31:43Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2012-12 | - |
dc.identifier.issn | 0950-1991 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/164048 | - |
dc.description.abstract | Genomic reprogramming factors in the cytoplasm of germinal vesicle (GV) stage oocytes have been shown to improve the efficiency of producing cloned mouse offspring through the exposure of nuclei to a GV cytoplasmic extract prior to somatic cell nuclear transfer (SCNT) to enucleated oocytes. Here, we developed an extract of GV stage pig oocytes (GVcyto-extract) to investigate epigenetic reprogramming events in treated somatic cell nuclei. This extract induced differentiation-associated changes in fibroblasts, resulting in cells that exhibit pluripotent stem cell-like characteristics and that redifferentiate into three primary germ cell layers both in vivo and in vitro. The GVcyto-extract treatment induced large numbers of high-quality SCNT-generated blastocysts, with methylation and acetylation of H3-K9 and expression of Oct4 and Nanog at levels similar to in vitro fertilized embryos. Thus, GVcyto-extract could elicit differentiation plasticity in treated fibroblasts, and SCNT-mediated reprogramming reset the epigenetic state in treated cells more efficiently than in untreated cells. In summary, we provide evidence for the generation of stem-like cells from differentiated somatic cells by treatment with porcine GVcyto-extract. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | COMPANY BIOLOGISTS LTD | - |
dc.title | Epigenetic reprogramming in somatic cells induced by extract from germinal vesicle stage pig oocytes | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Paik, Seung-Sam | - |
dc.identifier.doi | 10.1242/dev.086116 | - |
dc.identifier.scopusid | 2-s2.0-84868545480 | - |
dc.identifier.wosid | 000310780300005 | - |
dc.identifier.bibliographicCitation | DEVELOPMENT, v.139, no.23, pp.4330 - 4340 | - |
dc.relation.isPartOf | DEVELOPMENT | - |
dc.citation.title | DEVELOPMENT | - |
dc.citation.volume | 139 | - |
dc.citation.number | 23 | - |
dc.citation.startPage | 4330 | - |
dc.citation.endPage | 4340 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Developmental Biology | - |
dc.relation.journalWebOfScienceCategory | Developmental Biology | - |
dc.subject.keywordPlus | XENOPUS-LAEVIS OOCYTE | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | NUCLEAR TRANSFER | - |
dc.subject.keywordPlus | PARTHENOGENETIC DIPLOIDS | - |
dc.subject.keywordPlus | MEIOTIC MATURATION | - |
dc.subject.keywordPlus | MOUSE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CHROMATIN | - |
dc.subject.keywordPlus | HISTONE | - |
dc.subject.keywordPlus | EMBRYOS | - |
dc.subject.keywordAuthor | Epigenetic reprogramming | - |
dc.subject.keywordAuthor | Histone methylation | - |
dc.subject.keywordAuthor | Nuclear transfer | - |
dc.subject.keywordAuthor | Reprogrammed cells | - |
dc.subject.keywordAuthor | Oocyte extract | - |
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