Loss of Mel-18 enhances breast cancer stem cell activity and tumorigenicity through activating Notch signaling mediated by the Wnt/TCF pathway
DC Field | Value | Language |
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dc.contributor.author | Won, Hee-Young | - |
dc.contributor.author | Lee, Jeong-Yeon | - |
dc.contributor.author | Shin, Dong-Hui | - |
dc.contributor.author | Park, Ji-Hye | - |
dc.contributor.author | Nam, Jeong-Seok | - |
dc.contributor.author | Kim, Hyoung-Chin | - |
dc.contributor.author | Kong, Gu | - |
dc.date.accessioned | 2022-07-16T12:32:22Z | - |
dc.date.available | 2022-07-16T12:32:22Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2012-12 | - |
dc.identifier.issn | 0892-6638 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/164056 | - |
dc.description.abstract | Mel-18 has been proposed as a negative regulator of Bmi-1, a cancer stem cell (CSC) marker, but it is still unclear whether Mel-18 is involved in CSC regulation. Here, we examined the effect of Mel-18 on the stemness of human breast CSCs. In Mel-18 small hairpin RNA (shRNA)-transduced MCF-7 cells, side population (SP) cells and breast CSC surface marker (CD44(+)/CD24(-)/ESA(+))-expressing cells, which imply a CSC population, were enriched. Moreover, the self-renewal of CSCs was enhanced by Mel-18 knockdown, as measured by the ability for tumorsphere formation in vitro and tumor-initiating capacity in vivo. Similarly, Mel-18 overexpression inhibited the number and self-renewal activity of breast CSCs in SK-BR-3 cells. Furthermore, our data showed that Mel-18 blockade up-regulated the expression of the Wnt/TCF target Jagged-1, a Notch ligand, and consequently activated the Notch pathway. Pharmacologic inhibition of the Notch and Wnt pathways abrogated Mel-18 knockdown-mediated tumorsphere formation ability. Taken together, our findings suggest that Mel-18 is a novel negative regulator of breast CSCs that inhibits the stem cell population and in vitro and in vivo self-renewal through the inactivation of Wnt-mediated Notch signaling.-Won, H.-Y., Lee, J.-Y., Shin, D.-H., Park, J.-H., Nam, J.-S., Kim, H.-C., Kong, G. Loss of Mel-18 enhances breast cancer stem cell activity and tumorigenicity through activating Notch signaling mediated by the Wnt/TCF pathway. FASEB J. 26, 5002-5013 (2012). www.fasebj.org | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | FEDERATION AMER SOC EXP BIOL | - |
dc.title | Loss of Mel-18 enhances breast cancer stem cell activity and tumorigenicity through activating Notch signaling mediated by the Wnt/TCF pathway | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Jeong-Yeon | - |
dc.contributor.affiliatedAuthor | Kong, Gu | - |
dc.identifier.doi | 10.1096/fj.12-209247 | - |
dc.identifier.scopusid | 2-s2.0-84870346423 | - |
dc.identifier.wosid | 000311838300022 | - |
dc.identifier.bibliographicCitation | FASEB JOURNAL, v.26, no.12, pp.5002 - 5013 | - |
dc.relation.isPartOf | FASEB JOURNAL | - |
dc.citation.title | FASEB JOURNAL | - |
dc.citation.volume | 26 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 5002 | - |
dc.citation.endPage | 5013 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Life Sciences & Biomedicine - Other Topics | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | POLYCOMB GROUP PROTEINS | - |
dc.subject.keywordPlus | SELF-RENEWAL | - |
dc.subject.keywordPlus | TUMOR-SUPPRESSOR | - |
dc.subject.keywordPlus | PROSTATE-CANCER | - |
dc.subject.keywordPlus | SIDE POPULATION | - |
dc.subject.keywordPlus | POOR-PROGNOSIS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | BMI1 | - |
dc.subject.keywordPlus | TARGET | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordAuthor | polycomb | - |
dc.subject.keywordAuthor | stemness | - |
dc.subject.keywordAuthor | self-renewal | - |
dc.subject.keywordAuthor | Jagged-1 | - |
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