Coxsackievirus B3 used as a gene therapy vector to express functional FGF2
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, DS | - |
dc.contributor.author | Kim, H | - |
dc.contributor.author | Shim, SH | - |
dc.contributor.author | Kim, C | - |
dc.contributor.author | Song, M | - |
dc.contributor.author | Kim, YH | - |
dc.contributor.author | Jung, YW | - |
dc.contributor.author | Nam, JH | - |
dc.date.accessioned | 2022-07-16T12:43:00Z | - |
dc.date.available | 2022-07-16T12:43:00Z | - |
dc.date.created | 2021-05-11 | - |
dc.date.issued | 2012-12 | - |
dc.identifier.issn | 0969-7128 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/164115 | - |
dc.description.abstract | Current gene therapies are predominantly based on a handful of viral vectors. The limited choice of delivery vectors has been one of the stumbling blocks to the advancement of gene therapy. Therefore, the development of novel recombinant vectors should facilitate the application of gene therapies. In this study, we examined coxsackievirus B3 (CVB3) as a novel recombinant vector for the delivery and expression of a foreign gene in vitro and in vivo. A recombinant CVB3 complementary DNA was constructed by inserting a gene encoding human fibroblast growth factor 2 (FGF2). The recombinant virus (CVB3-FGF2) efficiently expressed FGF2 in HeLa cells and human cardiomyocytes in vitro and in mouse hindlimbs in vivo. The injection of the recombinant virus into mice with ischemic hindlimbs protected the hindlimbs from ischemic necrosis. CVB3-FGF2 injection significantly improved the blood flow in the ischemic limbs for over 3 weeks compared with that in the phosphate-buffered saline- or CVB3-injected controls, suggesting that FGF2 expressed from CVB3-FGF2 is functional and therapeutically effective. The virulence of CVB3 was also drastically attenuated in the recombinant virus. Thus, CVB3 can be modified to express a functional foreign protein, supporting its use as a novel viral vector for gene therapy. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | Coxsackievirus B3 used as a gene therapy vector to express functional FGF2 | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, C | - |
dc.identifier.doi | 10.1038/gt.2011.201 | - |
dc.identifier.scopusid | 2-s2.0-84870795716 | - |
dc.identifier.wosid | 000311997800005 | - |
dc.identifier.bibliographicCitation | GENE THERAPY, v.19, no.12, pp.1159 - 1165 | - |
dc.relation.isPartOf | GENE THERAPY | - |
dc.citation.title | GENE THERAPY | - |
dc.citation.volume | 19 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 1159 | - |
dc.citation.endPage | 1165 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Genetics & Heredity | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Genetics & Heredity | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.subject.keywordPlus | ENDOTHELIAL-CELLS | - |
dc.subject.keywordPlus | CD31(+) CELLS | - |
dc.subject.keywordPlus | VIRAL VECTORS | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | PHENOTYPE | - |
dc.subject.keywordPlus | INFECTION | - |
dc.subject.keywordPlus | HINDLIMB | - |
dc.subject.keywordPlus | MUTATION | - |
dc.subject.keywordPlus | VARIANT | - |
dc.subject.keywordAuthor | coxsackievirus B3 | - |
dc.subject.keywordAuthor | viral vector | - |
dc.subject.keywordAuthor | human fibroblast growth factor 2 | - |
dc.identifier.url | https://www.nature.com/articles/gt2011201 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.