Deguelin, an Akt Inhibitor, Down-Regulates NF-kappa B Signaling and Induces Apoptosis in Colon Cancer Cells and Inhibits Tumor Growth in Mice
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kang, Hyoun Woo | - |
dc.contributor.author | Kim, Jung Mogg | - |
dc.contributor.author | Cha, Mi Yeon | - |
dc.contributor.author | Jung, Hyun Chae | - |
dc.contributor.author | Song, In Sung | - |
dc.contributor.author | Kim, Joo Sung | - |
dc.date.accessioned | 2022-07-16T12:56:50Z | - |
dc.date.available | 2022-07-16T12:56:50Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2012-11 | - |
dc.identifier.issn | 0163-2116 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/164295 | - |
dc.description.abstract | Deguelin, a naturally occurring rotenoid, is known to be an Akt inhibitor and to have an anti-tumor effect on several cancers. This study was performed to elucidate the effect of deguelin on apoptotic pathways related to NF-kappa B signaling in colon cancer cells and on the anti-tumor effect in colon cancer xenograft mice. We studied COLO 205 and HCT116 cells in the presence or absence of deguelin. NF-kappa B signaling was examined by real-time RT-PCR for interleukin (IL)-8, by Western blotting for I kappa B phosphorylation/degradation, and by the electrophoretic mobility shift assay. Cell death was determined by the MTT assay, and apoptosis by Annexin V-FITC staining and caspase-3 activity. We also assessed the expression of antiapoptotic and proapoptotic factors by use of RT-PCR. In colon cancer xenograft mice, we evaluated the effect of deguelin on inoculated tumor growth, and apoptotic index was measured by the in vivo TUNEL assay. Deguelin significantly inhibited IL-8 gene expression, I kappa B phosphorylation/degradation, and DNA binding activity of NF-kappa B in colon cancer cells. Deguelin induced cell death and apoptosis in colon cancer cells in a dose and time-dependent manner. Deguelin down-regulated expression of NF-kappa B-mediated antiapoptotic factors such as cFLIP, Bcl-2, and Bcl-X-L. In the colon cancer xenograft model, the volume of the tumor treated with deguelin was significantly lower than that of the control, and the apoptotic index for deguelin-treated mice was much higher. Deguelin might be a potential therapeutic agent for treatment of colorectal cancer. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER | - |
dc.title | Deguelin, an Akt Inhibitor, Down-Regulates NF-kappa B Signaling and Induces Apoptosis in Colon Cancer Cells and Inhibits Tumor Growth in Mice | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Jung Mogg | - |
dc.identifier.doi | 10.1007/s10620-012-2237-x | - |
dc.identifier.scopusid | 2-s2.0-84871574859 | - |
dc.identifier.wosid | 000309867800034 | - |
dc.identifier.bibliographicCitation | DIGESTIVE DISEASES AND SCIENCES, v.57, no.11, pp.2873 - 2882 | - |
dc.relation.isPartOf | DIGESTIVE DISEASES AND SCIENCES | - |
dc.citation.title | DIGESTIVE DISEASES AND SCIENCES | - |
dc.citation.volume | 57 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 2873 | - |
dc.citation.endPage | 2882 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Gastroenterology & Hepatology | - |
dc.relation.journalWebOfScienceCategory | Gastroenterology & Hepatology | - |
dc.subject.keywordPlus | INTESTINAL EPITHELIAL-CELLS | - |
dc.subject.keywordPlus | ACUTE MURINE COLITIS | - |
dc.subject.keywordPlus | CHEMOPREVENTIVE ACTIVITY | - |
dc.subject.keywordPlus | ORNITHINE-DECARBOXYLASE | - |
dc.subject.keywordPlus | DRUG DEVELOPMENT | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | TUMORIGENESIS | - |
dc.subject.keywordPlus | SUPPRESSION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordAuthor | Deguelin | - |
dc.subject.keywordAuthor | Colon cancer | - |
dc.subject.keywordAuthor | NF-kappa B | - |
dc.subject.keywordAuthor | Apoptosis | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.