Alveolar Macrophages Play a Key Role in Cockroach-Induced Allergic Inflammation via TNF-alpha Pathway
DC Field | Value | Language |
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dc.contributor.author | Kim, Joo Young | - |
dc.contributor.author | Sohn, Jung Ho | - |
dc.contributor.author | Choi, Je-Min | - |
dc.contributor.author | Lee, Jae-Hyun | - |
dc.contributor.author | Hong, Chein-Soo | - |
dc.contributor.author | Lee, Joo-Shil | - |
dc.contributor.author | Park, Jung-Won | - |
dc.date.accessioned | 2022-07-16T13:38:17Z | - |
dc.date.available | 2022-07-16T13:38:17Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2012-10 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/164598 | - |
dc.description.abstract | The activity of the serine protease in the German cockroach allergen is important to the development of allergic disease. The protease-activated receptor (PAR)-2, which is expressed in numerous cell types in lung tissue, is known to mediate the cellular events caused by inhaled serine protease. Alveolar macrophages express PAR-2 and produce considerable amounts of tumor necrosis factor (TNF)-alpha. We determined whether the serine protease in German cockroach extract (GCE) enhances TNF-alpha production by alveolar macrophages through the PAR-2 pathway and whether the TNF-alpha production affects GCE-induced pulmonary inflammation. Effects of GCE on alveolar macrophages and TNF-alpha production were evaluated using in vitro MH-S and RAW264.6 cells and in vivo GCE-induced asthma models of BALB/c mice. GCE contained a large amount of serine protease. In the MH-S and RAW264.7 cells, GCE activated PAR-2 and thereby produced TNF-alpha. In the GCE-induced asthma model, intranasal administration of GCE increased airway hyperresponsiveness (AHR), inflammatory cell infiltration, productions of serum immunoglobulin E, interleukin (IL)-5, IL-13 and TNF-alpha production in alveolar macrophages. Blockade of serine proteases prevented the development of GCE induced allergic pathologies. TNF-alpha blockade also prevented the development of such asthma-like lesions. Depletion of alveolar macrophages reduced AHR and intracellular TNF-alpha level in pulmonary cell populations in the GCE-induced asthma model. These results suggest that serine protease from GCE affects asthma through an alveolar macrophage and TNF-alpha dependent manner, reflecting the close relation of innate and adaptive immune response in allergic asthma model. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.title | Alveolar Macrophages Play a Key Role in Cockroach-Induced Allergic Inflammation via TNF-alpha Pathway | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Choi, Je-Min | - |
dc.identifier.doi | 10.1371/journal.pone.0047971 | - |
dc.identifier.scopusid | 2-s2.0-84867682725 | - |
dc.identifier.wosid | 000310050200054 | - |
dc.identifier.bibliographicCitation | PLOS ONE, v.7, no.10, pp.1 - 12 | - |
dc.relation.isPartOf | PLOS ONE | - |
dc.citation.title | PLOS ONE | - |
dc.citation.volume | 7 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 12 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | PROTEASE-ACTIVATED RECEPTOR-2 | - |
dc.subject.keywordPlus | AIRWAY EPITHELIAL-CELLS | - |
dc.subject.keywordPlus | INNATE IMMUNE-RESPONSE | - |
dc.subject.keywordPlus | GERMAN-COCKROACH | - |
dc.subject.keywordPlus | DUST-MITE | - |
dc.subject.keywordPlus | IL-8 EXPRESSION | - |
dc.subject.keywordPlus | LUNG-DISEASE | - |
dc.subject.keywordPlus | ASTHMA | - |
dc.subject.keywordPlus | EXPOSURE | - |
dc.subject.keywordPlus | SENSITIZATION | - |
dc.identifier.url | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0047971 | - |
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