Characterizing the neurite outgrowth inhibitory effect of Mani

Abstract

Mani (myelin-associated neurite-outgrowth inhibitor) protein is implicated in both axonal guidance and axonal regeneration after central nervous system (CNS) injury. Here, we applied a neurite outgrowth assay, coupled with a siRNA-driven investigation and immunocytochemistry, to unveil Mani's axonal outgrowth inhibitory effect in embryonic rat cortical primary neurons in vitro. We further demonstrate Mani's neuronal localization in comparison with a principal subunit, Cdc27, of the anaphase promoting complex (APC). Considering the protein structure of Mani obtained via a series of bio-computational studies, we propose a Cdc27-Mani-APC-related signalling pathway may be involved in CNS axon regeneration.

Structured summary of protein interactions:

MANI physically interacts with DAZAP2 by two hybrid (View interaction)

MANI physically interacts with FAM168A by two hybrid (View interaction)

MANI physically interacts with YPEL2 by two hybrid (View interaction)

MANI physically interacts with TMTC1 by two hybrid (View interaction)