Amphiphilic peptide carrier for the combined delivery of curcumin and plasmid DNA into the lungs
DC Field | Value | Language |
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dc.contributor.author | Park, Ji Hwan | - |
dc.contributor.author | Kim, Hyun Ah | - |
dc.contributor.author | Park, Jin Hyeong | - |
dc.contributor.author | Lee, Minhyung | - |
dc.date.accessioned | 2022-07-16T13:55:12Z | - |
dc.date.available | 2022-07-16T13:55:12Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2012-09 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/164787 | - |
dc.description.abstract | In this study, the R7L10 peptide, which is composed of a 7-arginine stretch and a 10-leucine stretch, was evaluated as a carrier for the combined delivery of curcumin and plasmid DNA (pDNA) into the lungs. Curcumin is a natural product with anti-inflammatory and anti-tumor effects. Curcumin-loaded R7L10 (R7L10-curucmin) was prepared by an oil-in-water (O/W) emulsion/solvent evaporation method. In vitro transfection showed that R7L10-curcumin had higher transfection efficiency than R7L10. Although R7L10-curcumin had lower transfection efficiency than polyethylenimine (25 kDa, PEI25k) and lipofectamine, R7L10-curcumin had lower cytotoxicity. In gel retardation assays and heparin competition assays, R7L10-curcumin formed a more stable complex with pDNA than R7L10. The intracellular curcumin delivery efficiency of R7L10-curcumin was higher than that of curcumin only. Furthermore, R7L10-curcumin more efficiently decreased TNF-alpha level in lipopolysaccharide (LPS)-activated Raw264.7 macrophage cells than curcumin only. For in vivo evaluation, pDNA/R7L10-curcumin complexes were administered into mouse lungs by intratracheal instillation. The results revealed that R7L10-curcumin delivered pDNA more efficiently than R7L10, poly-L-lysine (PLL), or PEI25k. In addition, R7L10-curcumin decreased TNF-alpha level in lung tissues in an acute lung injury mouse model. In contrast to PEI25k, R7L10-curcumin did not show liver toxicity after intravenous injection. These results suggest that R7L10-curcumin is a useful carrier for the combined delivery of curcumin and pDNA into the lungs. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | Amphiphilic peptide carrier for the combined delivery of curcumin and plasmid DNA into the lungs | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Minhyung | - |
dc.identifier.doi | 10.1016/j.biomaterials.2012.05.046 | - |
dc.identifier.scopusid | 2-s2.0-84863560200 | - |
dc.identifier.wosid | 000307148200024 | - |
dc.identifier.bibliographicCitation | BIOMATERIALS, v.33, no.27, pp.6542 - 6550 | - |
dc.relation.isPartOf | BIOMATERIALS | - |
dc.citation.title | BIOMATERIALS | - |
dc.citation.volume | 33 | - |
dc.citation.number | 27 | - |
dc.citation.startPage | 6542 | - |
dc.citation.endPage | 6550 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.subject.keywordPlus | HEME OXYGENASE-1 GENE | - |
dc.subject.keywordPlus | DEXAMETHASONE | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | POLYETHYLENIMINE | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordAuthor | DNA | - |
dc.subject.keywordAuthor | Gene transfer | - |
dc.subject.keywordAuthor | Gene expression | - |
dc.subject.keywordAuthor | Cell viability | - |
dc.subject.keywordAuthor | Inflammation | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S014296121200587X?via%3Dihub | - |
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