Delivery of two-step transcription amplification exendin-4 plasmid system with arginine-grafted bioreducible polymer in type 2 diabetes animal model
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Pyung-Hwan | - |
dc.contributor.author | Lee, Minhyung | - |
dc.contributor.author | Kim, Sung Wan | - |
dc.date.accessioned | 2022-07-16T14:26:44Z | - |
dc.date.available | 2022-07-16T14:26:44Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2012-08 | - |
dc.identifier.issn | 0168-3659 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/165019 | - |
dc.description.abstract | Exendin-4, glucagon-like peptide 1 (GLP-1) receptor agonist, is an exocrine hormone, which has potent insulinotropic actions similar to GLP-1 such as stimulating insulin biosynthesis, facilitating glucose concentration dependent insulin secretion, slowing gastric emptying, reducing food intake and stimulating beta-cell proliferation. Exendin-4, also, has a longer half-life than GLP-1, due to its resistance to degradation by dipeptidyl peptidase-IV (DPP-IV). In spite of its many advantages as a therapeutic agent for diabetes, its clinical application is still restricted. Thus, to improve the activity of exendin-4 in vivo, gene therapy system was developed as an alternative method. An exendin-4 expression system was constructed using the two-step transcription amplification (TSTA) system, which is composed of p beta-Gal4-p65 and pUAS-SP-exendin-4 with combining the advantages of signal peptide (SP) in order to facilitate its secretion in ectopic cells or tissue. Arginine-grafted cyctaminebisacrylamide-diaminohexane polymer (ABP) was used as a gene carrier. Increased expression of exendin-4, glucose dependent insulin secretion in NIT-1 insulinoma cells, and high insulin expression in the presence of DPP-IV were evaluated in vitro after delivery of ABP/TSTA-SP-exendin-4. Blood glucose levels in diabetic mice were decreased dramatically from the third day for experimental period after single intravenous administration with ABP/TSTA-SP-exendin-4. The highest insulinotropic effect of exendin-4 was also observed in the ABP/TSTA/SP-exendin-4-treated mice groups, compared with the others groups from the 3rd day after injection. TSTA exendin-4 expression system with SP and ABP polymer has a potential gene therapy for the treatment of type 2 diabetes. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER | - |
dc.title | Delivery of two-step transcription amplification exendin-4 plasmid system with arginine-grafted bioreducible polymer in type 2 diabetes animal model | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Minhyung | - |
dc.identifier.doi | 10.1016/j.jconrel.2012.06.010 | - |
dc.identifier.scopusid | 2-s2.0-84864648267 | - |
dc.identifier.wosid | 000307769200002 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CONTROLLED RELEASE, v.162, no.1, pp.9 - 18 | - |
dc.relation.isPartOf | JOURNAL OF CONTROLLED RELEASE | - |
dc.citation.title | JOURNAL OF CONTROLLED RELEASE | - |
dc.citation.volume | 162 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 9 | - |
dc.citation.endPage | 18 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | DIPEPTIDYL PEPTIDASE-4 INHIBITORS | - |
dc.subject.keywordPlus | PEPTIDE-1 RECEPTOR AGONISTS | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | INCRETIN MIMETICS | - |
dc.subject.keywordPlus | GENE DELIVERY | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | EXENATIDE | - |
dc.subject.keywordPlus | GLP-1 | - |
dc.subject.keywordAuthor | Diabetes | - |
dc.subject.keywordAuthor | Exendin-4 polyplex | - |
dc.subject.keywordAuthor | TSTA system | - |
dc.subject.keywordAuthor | Gene delivery | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0168365912005032?via%3Dihub | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.