Heparin-coated superparamagnetic iron oxide for in vivo MR imaging of human MSCs
DC Field | Value | Language |
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dc.contributor.author | Lee, Ji-hye | - |
dc.contributor.author | Jung, Min Jin | - |
dc.contributor.author | Hwang, Yong Hwa | - |
dc.contributor.author | Lee, Young Jun | - |
dc.contributor.author | Lee, Seungsoo | - |
dc.contributor.author | Lee, Dong Yun | - |
dc.contributor.author | Shin, Heungsoo | - |
dc.date.accessioned | 2022-07-16T15:09:04Z | - |
dc.date.available | 2022-07-16T15:09:04Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2012-06 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/165417 | - |
dc.description.abstract | Human mesenchymal stem cells (hMSCs) offer significant therapeutic potential in the field of regenerative medicine and high-resolution magnetic resonance imaging (MRI) is useful modality to visualize in vivo kinetics of transplanted stem cells. For successful MR imaging, there is a great need for effective contrast agents for stem cell labeling with high uptake yield and low toxicity. Here, we present superparamagnetic iron oxide (SPIO) nanoparticles coated with unfractionated heparin (UFH-SPIO) as a new negative contrast agent for in vivo MR imaging of hMSCs. The uptake of UFH-SPIO by hMSCs was effective without the aid of transfection agents, which was dependent on the concentration and exposure time. The uptake efficiency of UFH-SPIO was greater than that of DEX-SPIO (SPIO coated with dextran) by approximately 3 folds when treated for 1 h. TEM and Prussian blue staining confirmed that UFH-SPIO nanoparticles were internalized into the cytosol of hMSCs which existed during in vitro subculture for 28 days. Low temperature endocytosis inhibition assay demonstrated that the incorporation of UFH-SPIO into hMSCs was likely to be mediated by endocytosis. When the phantom of UFH-SPIO-labeled hMSCs was visualized with 3-T T-2-weighted MRI, the hypointensity signals of UFH-SPIO-labeled hMSCs were linearly correlated with the concentration of the nanoparticles. The cellular labeling using UFH-SPIO did not reduce the viability, proliferation or differentiation potential to osteogenic and adipogenic lineages of hMSCs. When the UFH-SPIO-labeled hMSCs were transplanted into the left renal subcapsular membranes of nude mice, they were successfully visualized and detected by T-2 and T-2*-weighted MRI for a month. Collectively, these results suggest that UFH-SPIO nanoparticles are promising as a new MRI contrast agent for in vivo long-term tracking of hMSCs. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | Heparin-coated superparamagnetic iron oxide for in vivo MR imaging of human MSCs | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Dong Yun | - |
dc.contributor.affiliatedAuthor | Shin, Heungsoo | - |
dc.identifier.doi | 10.1016/j.biomaterials.2012.03.035 | - |
dc.identifier.scopusid | 2-s2.0-84859870962 | - |
dc.identifier.wosid | 000303966100010 | - |
dc.identifier.bibliographicCitation | BIOMATERIALS, v.33, no.19, pp.4861 - 4871 | - |
dc.relation.isPartOf | BIOMATERIALS | - |
dc.citation.title | BIOMATERIALS | - |
dc.citation.volume | 33 | - |
dc.citation.number | 19 | - |
dc.citation.startPage | 4861 | - |
dc.citation.endPage | 4871 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.subject.keywordPlus | MESENCHYMAL STEM-CELLS | - |
dc.subject.keywordPlus | TRANSFERRIN RECEPTOR | - |
dc.subject.keywordPlus | CONTRAST AGENTS | - |
dc.subject.keywordPlus | DRUG-DELIVERY | - |
dc.subject.keywordPlus | STROMAL CELLS | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | TRACKING | - |
dc.subject.keywordPlus | FERUMOXIDES | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | FIBROBLASTS | - |
dc.subject.keywordAuthor | Stem cell | - |
dc.subject.keywordAuthor | MRI (magnetic resonance imaging) | - |
dc.subject.keywordAuthor | Superparamagnetic iron oxide (SPIO) | - |
dc.subject.keywordAuthor | Heparin | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0142961212003225?via%3Dihub | - |
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