Phospholipase D1 is an Important Regulator of bFGF-Induced Neurotrophin-3 Expression and Neurite Outgrowth in H19-7 Cells
DC Field | Value | Language |
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dc.contributor.author | Choi, Hye-Jin | - |
dc.contributor.author | Chang, Brian Junho | - |
dc.contributor.author | Han, Joong-Soo | - |
dc.date.accessioned | 2022-07-16T15:10:19Z | - |
dc.date.available | 2022-07-16T15:10:19Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2012-06 | - |
dc.identifier.issn | 0893-7648 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/165432 | - |
dc.description.abstract | The purpose of this study was to examine the role of phospholipase D1 (PLD1) in basic fibroblast growth factor (bFGF)-induced neurotrophin-3 (NT-3) expression and neurite outgrowth in H19-7 rat hippocampal neuronal progenitor cells. Overexpression of PLD1 increased bFGF-induced NT-3 expression, and dominant-negative-PLD1 or PLD1 siRNA abolished bFGF-induced NT-3 expression and neurite outgrowth. Treatment with bFGF activated the RhoA/Rho-associated kinase (ROCK)/c-jun N-terminal kinase (JNK) pathway, and bFGF-induced NT-3 expression was blocked by a dominant-negative RhoA as well as by a specific Rho-kinase inhibitor (Y27632) and a SAPK/JNK inhibitor (SP600125). Furthermore, bFGF-induced JNK activation was also blocked by Y27632. These results indicate that the RhoA/ROCK/JNK pathway acts as an upstream signaling pathway in bFGF-induced NT-3 expression. Also, phosphatidic acid, the product of PLD, increased NT-3 expression. We found that PLD regulated the RhoA/ROCK/JNK pathway, which then led to Elk-1 transactivation. When Elk-1 activity was blocked by Elk-1 siRNA, bFGF-induced NT-3 expression and neurite outgrowth decreased. NT-3 overexpression increased neurite outgrowth, indicating that NT-3 is important for neurite outgrowth. Taken together, these results suggest that PLD1 is an important regulator of bFGF-induced NT-3 expression and neurite outgrowth, which are mediated by the RhoA/ROCK/JNK pathway via Elk-1 in H19-7 cells. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER | - |
dc.title | Phospholipase D1 is an Important Regulator of bFGF-Induced Neurotrophin-3 Expression and Neurite Outgrowth in H19-7 Cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Han, Joong-Soo | - |
dc.identifier.doi | 10.1007/s12035-012-8268-7 | - |
dc.identifier.scopusid | 2-s2.0-84867471757 | - |
dc.identifier.wosid | 000305559300010 | - |
dc.identifier.bibliographicCitation | MOLECULAR NEUROBIOLOGY, v.45, no.3, pp.507 - 519 | - |
dc.relation.isPartOf | MOLECULAR NEUROBIOLOGY | - |
dc.citation.title | MOLECULAR NEUROBIOLOGY | - |
dc.citation.volume | 45 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 507 | - |
dc.citation.endPage | 519 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Neurosciences & Neurology | - |
dc.relation.journalWebOfScienceCategory | Neurosciences | - |
dc.subject.keywordPlus | CEREBELLAR GRANULE NEURONS | - |
dc.subject.keywordPlus | INDUCED BCL-2 EXPRESSION | - |
dc.subject.keywordPlus | COMPLEX FACTOR ELK-1 | - |
dc.subject.keywordPlus | NEURAL STEM-CELLS | - |
dc.subject.keywordPlus | N-TERMINAL KINASE | - |
dc.subject.keywordPlus | SIGNAL-TRANSDUCTION | - |
dc.subject.keywordPlus | DENDRITIC GROWTH | - |
dc.subject.keywordPlus | PROTEIN-KINASES | - |
dc.subject.keywordPlus | NERVOUS-SYSTEM | - |
dc.subject.keywordPlus | MAP KINASES | - |
dc.subject.keywordAuthor | Phospholipase D1 | - |
dc.subject.keywordAuthor | RhoA | - |
dc.subject.keywordAuthor | JNK | - |
dc.subject.keywordAuthor | Elk-1 | - |
dc.subject.keywordAuthor | NT-3 | - |
dc.subject.keywordAuthor | Neurite outgrowth | - |
dc.identifier.url | https://link.springer.com/article/10.1007%2Fs12035-012-8268-7 | - |
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