Efficient enhancement of lentiviral transduction efficiency in murine spermatogonial stem cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Bang-Jin | - |
dc.contributor.author | Kim, Ki-Jung | - |
dc.contributor.author | Kim, Yong-Hee | - |
dc.contributor.author | Lee, Yong-An | - |
dc.contributor.author | Kim, Byung-Gak | - |
dc.contributor.author | Cho, Chul Min | - |
dc.contributor.author | Kang, Hye-Ryeon | - |
dc.contributor.author | Kim, Chul Geun | - |
dc.contributor.author | Ryu, Buom-Yong | - |
dc.date.accessioned | 2022-07-16T15:32:56Z | - |
dc.date.available | 2022-07-16T15:32:56Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2012-05 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/165705 | - |
dc.description.abstract | Spermatogonial stem cells (SSCs) are the foundation of spermatogenesis throughout postnatal life in male and have the ability to transmit genetic information to the subsequent generation. In this study, we have optimized the transduction efficiency of SSCs using a lentiviral vector by considering different multiplicity of infection (MOI), duration of infection, presence or absence of feeder layer and polycationic agents. We tested MOI of 5, 10 or 20 and infection duration of 6, 9 or 12 h respectively. After infection, cells were cultured for 1 week and as a result, the number of transduced SSCs increased significantly for MOI of 5 and 10 with 6 h of infection. When the same condition (MOI of 5 with 6 hours) was applied in presence or absence of STO feeder layer and infected SSCs were cultured for 3 weeks on the STO feeder layer, a significant increase in the number of transduced cells was observed for without the feeder layer during infection. We subsequently studied the effects of polycationic agents, polybrene and dioctadecylamidoglycyl spermine (DOGS), on the transduction efficiency. Compared with the polybrene treatment, the recovery rate of the transduced SSCs was significantly higher for the DOGS treatment. Therefore, our optimization study could contribute to the enhancement of germ-line modification of SSCs using lentiviral vectors and in generation of transgenic animals. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC MOLECULAR & CELLULAR BIOLOGY | - |
dc.title | Efficient enhancement of lentiviral transduction efficiency in murine spermatogonial stem cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Chul Geun | - |
dc.identifier.doi | 10.1007/s10059-012-2167-7 | - |
dc.identifier.scopusid | 2-s2.0-84863452603 | - |
dc.identifier.wosid | 000304176600004 | - |
dc.identifier.bibliographicCitation | MOLECULES AND CELLS, v.33, no.5, pp.449 - 455 | - |
dc.relation.isPartOf | MOLECULES AND CELLS | - |
dc.citation.title | MOLECULES AND CELLS | - |
dc.citation.volume | 33 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 449 | - |
dc.citation.endPage | 455 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001660802 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | FUNCTIONAL-CHARACTERISTICS | - |
dc.subject.keywordPlus | GERMLINE TRANSMISSION | - |
dc.subject.keywordPlus | TRANSGENIC RATS | - |
dc.subject.keywordPlus | SELF-RENEWAL | - |
dc.subject.keywordPlus | MOUSE TESTIS | - |
dc.subject.keywordPlus | TRANSPLANTATION | - |
dc.subject.keywordPlus | LIPOSOMES | - |
dc.subject.keywordAuthor | germline modification | - |
dc.subject.keywordAuthor | Lentivirus | - |
dc.subject.keywordAuthor | polycationic agent | - |
dc.subject.keywordAuthor | spermatogonial stem cell | - |
dc.subject.keywordAuthor | transgenesis | - |
dc.identifier.url | https://link.springer.com/article/10.1007/s10059-012-2167-7 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.