4-Octylphenol induces developmental abnormalities and interferes the differentiation of neural crest cells in Xenopus laevis embryos
DC Field | Value | Language |
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dc.contributor.author | Xu, Y. | - |
dc.contributor.author | Jang, J.H. | - |
dc.contributor.author | Gye, M.C. | - |
dc.date.accessioned | 2021-07-30T04:51:40Z | - |
dc.date.available | 2021-07-30T04:51:40Z | - |
dc.date.created | 2021-05-13 | - |
dc.date.issued | 2021 | - |
dc.identifier.issn | 0269-7491 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/1658 | - |
dc.description.abstract | Developmental toxicity of 4-octylphenol (OP), an estrogenic endocrine disruptor was verified using frog embryo teratogenesis assay Xenopus. LC50, EC50Malformtion and EC50Melanocyte-dysgenesis of OP were 9.9, 10.5, and 2.4 μM, respectively. In tadpoles, despite the low teratogenic index, 2 μM OP significantly inhibited head cartilage development and tail malformation. The total length of tadpole was significantly increased at 5 μM and decreased at 10 μM OP. In OP-treated tadpoles, head cartilages were frequently missed and col2a1 mRNA was decreased at 2 μM, indicating a chondrogenic defect in developing head. In the head skin of 1 μM OP-treated tadpoles, number of melanocytes and melanogenic pathway genes expression were significantly decreased. In the head-neck junction of stage 22 embryos, OP increased foxd3 and sox10 mRNA and SOX10(+) neural crest cells (NCCs) in somite mesoderm and endoderm, indicating the inhibition of chondrogenic differentiation, ectopic migration to endoderm, and undifferentiation of NCCs by OP. Together, OP-induced head dysplasia and inhibition of melanogenesis may be attributable to deregulation of neural crest cells in embryos. In tadpoles, OP at 1 μM significantly increased lipid hydroperoxide and induced spliced xbp1 mRNA, an IRE1 pathway endoplasmic reticulum stress (ERS) marker and p-eIF2α protein, a PERK pathway ERS marker. OP at 10 μM induced CHOP mRNA, pro-apoptotic genes expression, DNA fragmentation, and cleaved caspase-3, suggesting that OP differentially induced ERS and apoptosis according to the concentration in embryos. In 5–10 μM OP-treated stage 22 embryos and stage 45 tadpole heads, Ki67 was significantly increased, suggesting the apoptosis-induced proliferation of embryonic cells in the OP-treated embryos. Together, OP should be managed as a developmental toxicant altering the behavior of NCCs in vertebrates. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | 4-Octylphenol induces developmental abnormalities and interferes the differentiation of neural crest cells in Xenopus laevis embryos | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Gye, M.C. | - |
dc.identifier.doi | 10.1016/j.envpol.2021.116560 | - |
dc.identifier.scopusid | 2-s2.0-85099934530 | - |
dc.identifier.wosid | 000625379400059 | - |
dc.identifier.bibliographicCitation | Environmental Pollution, v.274, pp.1 - 15 | - |
dc.relation.isPartOf | Environmental Pollution | - |
dc.citation.title | Environmental Pollution | - |
dc.citation.volume | 274 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 15 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Environmental Sciences & Ecology | - |
dc.relation.journalWebOfScienceCategory | Environmental Sciences | - |
dc.subject.keywordPlus | Cartilage | - |
dc.subject.keywordPlus | Cell death | - |
dc.subject.keywordPlus | Chondrogenic differentiation | - |
dc.subject.keywordPlus | Developmental toxicity | - |
dc.subject.keywordPlus | Endoplasmic reticulum stress | - |
dc.subject.keywordPlus | Estrogenic endocrine disruptors | - |
dc.subject.keywordPlus | Frog embryo teratogenesis assay Xenopus | - |
dc.subject.keywordPlus | Lipid hydroperoxide | - |
dc.subject.keywordPlus | Neural crest cells | - |
dc.subject.keywordPlus | Pro-apoptotic genes | - |
dc.subject.keywordPlus | Genes | - |
dc.subject.keywordPlus | 4 octylphenol | - |
dc.subject.keywordPlus | caspase 3 | - |
dc.subject.keywordPlus | initiation factor 2alpha | - |
dc.subject.keywordPlus | lipid hydroperoxide | - |
dc.subject.keywordPlus | messenger RNA | - |
dc.subject.keywordPlus | animal cell | - |
dc.subject.keywordPlus | animal tissue | - |
dc.subject.keywordPlus | Article | - |
dc.subject.keywordPlus | cell differentiation | - |
dc.subject.keywordPlus | cell migration | - |
dc.subject.keywordPlus | chondrogenesis | - |
dc.subject.keywordPlus | controlled study | - |
dc.subject.keywordPlus | DNA fragmentation | - |
dc.subject.keywordPlus | embryo | - |
dc.subject.keywordPlus | embryo cell | - |
dc.subject.keywordPlus | endoderm | - |
dc.subject.keywordPlus | endoplasmic reticulum stress | - |
dc.subject.keywordPlus | foxd3 gene | - |
dc.subject.keywordPlus | gene | - |
dc.subject.keywordPlus | gene expression | - |
dc.subject.keywordPlus | histology | - |
dc.subject.keywordPlus | lipid peroxidation | - |
dc.subject.keywordPlus | melanocyte | - |
dc.subject.keywordPlus | melanogenesis | - |
dc.subject.keywordPlus | mesoderm | - |
dc.subject.keywordPlus | neural crest cell | - |
dc.subject.keywordPlus | nonhuman | - |
dc.subject.keywordPlus | protein cleavage | - |
dc.subject.keywordPlus | signal transduction | - |
dc.subject.keywordPlus | sox10 gene | - |
dc.subject.keywordPlus | tadpole | - |
dc.subject.keywordPlus | Xenopus laevis | - |
dc.subject.keywordPlus | Vertebrata | - |
dc.subject.keywordPlus | Xenopus laevis | - |
dc.subject.keywordAuthor | 4-Octylphenol | - |
dc.subject.keywordAuthor | Head dysgenesis | - |
dc.subject.keywordAuthor | Melanogenic defect | - |
dc.subject.keywordAuthor | Neural crest cells | - |
dc.subject.keywordAuthor | Xenopus laevis embryos | - |
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