Glutaredoxin 2a, a mitochondrial isoform, plays a protective role in a human cell line under serum deprivation
- Authors
- Kim, Su-Jung; Jung, Hyun-Joo; Choi, Hojin; Lim, Chang-Jin
- Issue Date
- Apr-2012
- Publisher
- SPRINGER
- Keywords
- Glutaredoxin; Reactive oxygen species; Serum deprivation; Apoptosis
- Citation
- MOLECULAR BIOLOGY REPORTS, v.39, no.4, pp.3755 - 3765
- Indexed
- SCIE
SCOPUS
- Journal Title
- MOLECULAR BIOLOGY REPORTS
- Volume
- 39
- Number
- 4
- Start Page
- 3755
- End Page
- 3765
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/165915
- DOI
- 10.1007/s11033-011-1152-0
- ISSN
- 0301-4851
- Abstract
- The roles of mitochondrial glutaredoxin (Grx2a) under serum deprivation were assessed using the human stable HepG2 cell lines overexpressing or down-regulating Grx2a. The Grx2a-overexpressing stable cells displayed enhanced proliferation, decreased reactive oxygen species (ROS) and caspase-3 activity levels, and increased total GSH level, compared to the vector control cells. These characteristics of the overexpressing stable cells were reversed by down-regulating Grx2a in the same cell line. In the limited serum conditions, the Grx2a-overexpressing stable pcDNA3.0/HA-Grx2a cells exhibited higher cellular viabilities and total GSH level, and showed much lower enhancement in ROS and caspase-3 activity levels than the vector control pcDNA3.0/HA cells. However, the Grx2a-down-regulating stable cells gave rise to diminished cellular viabilities and further decreased total GSH level, and contained significantly higher ROS and caspase-3 activity levels, under serum deprivation than the vector control cells. These results suggest that Grx2a plays proliferative and anti-apoptotic roles under serum deprivation.
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