The CD49d(+/high) subpopulation from isolated human breast sarcoma spheres possesses tumor-initiating ability
DC Field | Value | Language |
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dc.contributor.author | Lee, Kyung-Min | - |
dc.contributor.author | Han, Wonshik | - |
dc.contributor.author | Kim, Jong Bin | - |
dc.contributor.author | Shin, Incheol | - |
dc.contributor.author | Ko, Eunyoung | - |
dc.contributor.author | Park, In Ae | - |
dc.contributor.author | Lee, Dong Sup | - |
dc.contributor.author | Oh, Kuenhee | - |
dc.contributor.author | Noh, Dung-Young | - |
dc.date.accessioned | 2022-07-16T16:18:35Z | - |
dc.date.available | 2022-07-16T16:18:35Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2012-03 | - |
dc.identifier.issn | 1019-6439 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/166114 | - |
dc.description.abstract | Primary breast sarcomas (PBSs) that arise from mammary stroma are very rare, highly aggressive and therapy-resistant tumors with a heterogeneous phenotype. In this study, we sought to identify tumor-initiating cells (TICs) in PBSs and to describe their features. We isolated long-term self-renewing sarcospheres (designated NDY-1) from primary breast carcinosarcoma tissue (sarcoma component >95%) using the anchorage-independent culture method. NDY-1 spheres expressed various mesenchymal cell markers, and their tumorigenic potential was markedly reduced in adherent culture conditions, compared to spheres. Screening for integrins revealed a marked decrease in CD49d expression in adherent culture conditions of NDY-1. The CD49d(+/high) subpopulation sorted from NDY-1 spheres displayed higher cell viability and sphere-forming ability than CD49d(-/low) population in vitro. Moreover, the CD49d(+/high) population displayed high tumor initiating ability in limiting dilution transplantation to NOD/SCID mice, and the xenotransplanted CD49d(+/high) population recapitulated the complexity of the original primary tumors. Greater doxorubicin resistance was exhibited by the CD49d(+/high) population, compared with the CD49d(-/low) population. Thus, our results collectively demonstrate that CD49d(+/high) cells from sarcospheres display enhanced sphere-forming, drug resistance and tumor-initiating abilities. To our knowledge, this is the first study to identify TICs from breast sarcoma. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SPANDIDOS PUBL LTD | - |
dc.title | The CD49d(+/high) subpopulation from isolated human breast sarcoma spheres possesses tumor-initiating ability | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Shin, Incheol | - |
dc.identifier.doi | 10.3892/ijo.2011.1289 | - |
dc.identifier.scopusid | 2-s2.0-84863116872 | - |
dc.identifier.wosid | 000300118200006 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF ONCOLOGY, v.40, no.3, pp.665 - 672 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF ONCOLOGY | - |
dc.citation.title | INTERNATIONAL JOURNAL OF ONCOLOGY | - |
dc.citation.volume | 40 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 665 | - |
dc.citation.endPage | 672 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.subject.keywordPlus | CANCER STEM-CELLS | - |
dc.subject.keywordPlus | DRUG-RESISTANCE | - |
dc.subject.keywordPlus | INTEGRINS | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | ADHESION | - |
dc.subject.keywordPlus | BIOLOGY | - |
dc.subject.keywordPlus | TUMORIGENESIS | - |
dc.subject.keywordPlus | PROGRESSION | - |
dc.subject.keywordPlus | CARCINOMA | - |
dc.subject.keywordAuthor | tumor-initiating cells | - |
dc.subject.keywordAuthor | breast sarcoma | - |
dc.subject.keywordAuthor | CD49d | - |
dc.identifier.url | https://www.spandidos-publications.com/10.3892/ijo.2011.1289 | - |
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