Effect of glucagon-like peptide-1 gene expression on graft function in mouse islet transplantation
DC Field | Value | Language |
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dc.contributor.author | Chae, Hee Young | - |
dc.contributor.author | Kang, Jun Goo | - |
dc.contributor.author | Kim, Chul Sik | - |
dc.contributor.author | Lee, Seong Jin | - |
dc.contributor.author | Lee, Minhyung | - |
dc.contributor.author | Kang, Dongchul | - |
dc.contributor.author | Jun, Hee-Sook | - |
dc.contributor.author | Ihm, Sung-Hee | - |
dc.date.accessioned | 2022-07-16T16:47:07Z | - |
dc.date.available | 2022-07-16T16:47:07Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2012-02 | - |
dc.identifier.issn | 0934-0874 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/166333 | - |
dc.description.abstract | This study investigated the effect of local glucagon-like peptide-1 (GLP-1) production within mouse islets on cytoprotection in vitro and in vivo by gene transfer of GLP-1. Transduction of recombinant adenovirus vector expressing GLP-1 (rAd-GLP-1) induced a significant increase in bioactive GLP-1 in the mouse islet culture, whereas transduction with adenovirus vector expressing beta-galactosidase (rAd-LacZ), as a control, had no effect on GLP-1 secretion. Islets transduced with rAd-GLP-1 were protected from H2O2-induced cell damage in vitro. In addition, glucose-stimulated insulin secretion was significantly increased in rAd-GLP-1-transduced islets. When transplanted under the kidney capsule of diabetic syngeneic mice, islet grafts retrieved 4 or 7 days after transplantation revealed that the rAd-GLP-1-transduced group had significantly more Ki67-positive cells as compared with the rAd-LacZ-transduced group. Regarding blood glucose control, diabetic mice transplanted with a marginal mass of rAd-GLP-1-transduced islets became normoglycemic more rapidly and 78% of the recipients were normoglycemic at 35 days post-transplant, whereas only 48% of the mice transplanted with rAd-LacZ-transduced islets were normoglycemic (P < 0.05). In conclusion, delivery of the GLP-1 gene to islets enhanced islet cell survival during the early post-transplant period, and preserved islet mass and functions over time in the transplants. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.title | Effect of glucagon-like peptide-1 gene expression on graft function in mouse islet transplantation | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Minhyung | - |
dc.identifier.doi | 10.1111/j.1432-2277.2011.01394.x | - |
dc.identifier.scopusid | 2-s2.0-84855987526 | - |
dc.identifier.wosid | 000299103800016 | - |
dc.identifier.bibliographicCitation | TRANSPLANT INTERNATIONAL, v.25, no.2, pp.242 - 249 | - |
dc.relation.isPartOf | TRANSPLANT INTERNATIONAL | - |
dc.citation.title | TRANSPLANT INTERNATIONAL | - |
dc.citation.volume | 25 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 242 | - |
dc.citation.endPage | 249 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Surgery | - |
dc.relation.journalResearchArea | Transplantation | - |
dc.relation.journalWebOfScienceCategory | Surgery | - |
dc.relation.journalWebOfScienceCategory | Transplantation | - |
dc.subject.keywordPlus | EXENATIDE SYNTHETIC EXENDIN-4 | - |
dc.subject.keywordPlus | HUMAN PANCREATIC-ISLETS | - |
dc.subject.keywordPlus | BETA-CELL MASS | - |
dc.subject.keywordPlus | DIABETIC-RATS | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | GLP-1 | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordAuthor | glucagon-like peptide-1 (GLP-1) | - |
dc.subject.keywordAuthor | islet | - |
dc.subject.keywordAuthor | transplantation | - |
dc.identifier.url | https://onlinelibrary.wiley.com/doi/10.1111/j.1432-2277.2011.01394.x | - |
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