Dexamethasone-loaded peptide micelles for delivery of the heme oxygenase-1 gene to ischemic brain
DC Field | Value | Language |
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dc.contributor.author | Lee, Jiyoung | - |
dc.contributor.author | Hyun, Hyesun | - |
dc.contributor.author | Kim, Jinyoung | - |
dc.contributor.author | Ryu, Jae Hwan | - |
dc.contributor.author | Kim, Hyun Ah | - |
dc.contributor.author | Park, Ji Hwan | - |
dc.contributor.author | Lee, Minhyung | - |
dc.date.accessioned | 2022-07-16T16:53:43Z | - |
dc.date.available | 2022-07-16T16:53:43Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2012-02 | - |
dc.identifier.issn | 0168-3659 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/166404 | - |
dc.description.abstract | The R3V6 peptides, which are composed of a 3-arginine block and a 6-valine block, formed self-assembled micelles in aqueous solution. Dye quenching assays showed that a hydrophobic fluorescent dye, 5-dodecanoylaminofluorescein (DAF), interacted with and was loaded into the hydrophobic core of the micelles. In this study, dexamethasone-loaded R3V6 peptide micelles (R3V6-Dexa) were evaluated as a gene carrier. R3V6-Dexa had higher gene delivery efficiency in human embryonic kidney 293 cells compared to those of the R3V6 peptides and poly-L-lysine (PLL). Dexamethasone might stabilize the micelle structure of the R3V6 peptides by forming strong hydrophobic cores and enhanced the transfection efficiency. Furthermore, R3V6-Dexa reduced the expression of an inflammatory cytokine, interleukin-6 (IL-6), more efficiently in lipopolysaccharide (LPS)-induced Raw264.7 cells than did dexamethasone, suggesting that R3V6-Dexa is also a useful carrier for dexamethasone delivery. A focal brain ischemia-reperfusion model was produced by middle cerebral artery occlusion (MCAO). A heme oxygenase-1 (HO-1) expression plasmid DNA, pSV-HO-1, was delivered into the brain using R3V6-Dexa as a carrier. The pSV-HO-1/R3V6-Dexa complex was injected into the brain 1 hr prior to MCAO. Twenty-four hours later, the HO-1 expression of the pSV-HO-1/R3V6-Dexa injection group was higher than those of the MCAO control, p beta-Luc/R3V6-Dexa, and pSV-HO-1/PEI25k injection groups. In addition, the infarct size was reduced due to the delivery of pSV-HO-1/R3V6-Dexa complex. Therefore, R3V6-Dexa may be a useful carrier for HO-1 gene delivery and stroke gene therapy. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER | - |
dc.title | Dexamethasone-loaded peptide micelles for delivery of the heme oxygenase-1 gene to ischemic brain | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Minhyung | - |
dc.identifier.doi | 10.1016/j.jconrel.2011.11.001 | - |
dc.identifier.scopusid | 2-s2.0-84857922008 | - |
dc.identifier.wosid | 000302605900016 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CONTROLLED RELEASE, v.158, no.1, pp.131 - 138 | - |
dc.relation.isPartOf | JOURNAL OF CONTROLLED RELEASE | - |
dc.citation.title | JOURNAL OF CONTROLLED RELEASE | - |
dc.citation.volume | 158 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 131 | - |
dc.citation.endPage | 138 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | LOW-MOLECULAR-WEIGHT | - |
dc.subject.keywordPlus | RICH PEPTIDES | - |
dc.subject.keywordPlus | CARRIER | - |
dc.subject.keywordPlus | POLYETHYLENIMINE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | INJURY | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | LIPOPOLYMER | - |
dc.subject.keywordPlus | PROTECTION | - |
dc.subject.keywordPlus | VIGILANT | - |
dc.subject.keywordAuthor | Gene delivery | - |
dc.subject.keywordAuthor | Gene therapy | - |
dc.subject.keywordAuthor | Peptide | - |
dc.subject.keywordAuthor | Micelle | - |
dc.subject.keywordAuthor | Stroke | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0168365911010315?via%3Dihub | - |
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