MiR-200b is involved in Tgf-beta signaling to regulate mammalian palate development
DC Field | Value | Language |
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dc.contributor.author | Shin, Jeong-Oh | - |
dc.contributor.author | Lee, Jong-Min | - |
dc.contributor.author | Cho, Kyoung-Won | - |
dc.contributor.author | Kwak, Sungwook | - |
dc.contributor.author | Kwon, Hyuk-Jae | - |
dc.contributor.author | Lee, Min-Jung | - |
dc.contributor.author | Cho, Sung-Won | - |
dc.contributor.author | Kim, Kye-Seong | - |
dc.contributor.author | Jung, Han-Sung | - |
dc.date.accessioned | 2022-07-16T17:06:11Z | - |
dc.date.available | 2022-07-16T17:06:11Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2012-01 | - |
dc.identifier.issn | 0948-6143 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/166524 | - |
dc.description.abstract | Various cellular and molecular events are involved in palatogenesis, including apoptosis, epithelialmesenchymal transition (EMT), cell proliferation, and cell migration. Smad2 and Snail, which are well-known key mediators of the transforming growth factor beta (Tgf-beta) pathway, play a crucial role in the regulation of palate development. Regulatory effects of microRNA 200b (miR-200b) on Smad2 and Snail in palatogenesis have not yet been elucidated. The aim of this study is to determine the relationship between palate development regulators miR-200b and Tgf-beta-mediated genes. Expression of miR-200b, E-cadherin, Smad2, and Snail was detected in the mesenchyme of the mouse palate, while miR-200b was expressed in the medial edge epithelium (MEE) and palatal mesenchyme. After the contact of palatal shelves, miR-200b was no longer expressed in the mesenchyme around the fusion region. The binding activity of miR-200b to both Smad2 and Snail was examined using a luciferase assay. MiR-200b directly targeted Smad2 and Snail at both cellular and molecular levels. The function of miR-200b was determined by overexpression via a lentiviral vector in the palatal shelves. Ectopic expression of miR-200b resulted in suppression of these Tgf-beta-mediated regulators and changes of apoptosis and cell proliferation in the palatal fusion region. These results suggest that miR-200b plays a crucial role in regulating the Smad2, Snail, and in apoptosis during palatogenesis by acting as a direct non-coding, influencing factor. Furthermore, the molecular interactions between miR-200b and Tgf-beta signaling are important for proper palatogenesis and especially for palate fusion. Elucidating the mechanism of palatogenesis may aid the design of effective gene-based therapies for the treatment of congenital cleft palate. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER | - |
dc.title | MiR-200b is involved in Tgf-beta signaling to regulate mammalian palate development | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Kye-Seong | - |
dc.identifier.doi | 10.1007/s00418-011-0876-1 | - |
dc.identifier.scopusid | 2-s2.0-84857631069 | - |
dc.identifier.wosid | 000299380200006 | - |
dc.identifier.bibliographicCitation | HISTOCHEMISTRY AND CELL BIOLOGY, v.137, no.1, pp.67 - 78 | - |
dc.relation.isPartOf | HISTOCHEMISTRY AND CELL BIOLOGY | - |
dc.citation.title | HISTOCHEMISTRY AND CELL BIOLOGY | - |
dc.citation.volume | 137 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 67 | - |
dc.citation.endPage | 78 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Microscopy | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Microscopy | - |
dc.subject.keywordPlus | EPITHELIAL-MESENCHYMAL TRANSITION | - |
dc.subject.keywordPlus | MEDIAL EDGE EPITHELIUM | - |
dc.subject.keywordPlus | E-CADHERIN | - |
dc.subject.keywordPlus | CLEFT-PALATE | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | REPRESSORS ZEB1 | - |
dc.subject.keywordPlus | CELL-MIGRATION | - |
dc.subject.keywordPlus | FUSION | - |
dc.subject.keywordPlus | SNAIL | - |
dc.subject.keywordAuthor | Palatogenesis | - |
dc.subject.keywordAuthor | MiR-200b | - |
dc.subject.keywordAuthor | Smad2 | - |
dc.subject.keywordAuthor | Snail | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | Cell proliferation | - |
dc.identifier.url | https://link.springer.com/article/10.1007/s00418-011-0876-1 | - |
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