Efficient GLP-1 gene delivery using two-step transcription amplification plasmid system with a secretion signal peptide and arginine-grafted bioreducible polymer
DC Field | Value | Language |
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dc.contributor.author | Kim, Tae-il | - |
dc.contributor.author | Lee, Minhyung | - |
dc.contributor.author | Kim, Sung Wan | - |
dc.date.accessioned | 2022-07-16T17:10:05Z | - |
dc.date.available | 2022-07-16T17:10:05Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2012-01 | - |
dc.identifier.issn | 0168-3659 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/166569 | - |
dc.description.abstract | Glucagon-like peptide (GLP-1) encoding dual plasmid (pDNA) system (TSTA (SP-GLP-1)) which is composed of p beta-Gal4-p65 and pUAS-SP-GLP-1 was constructed to improve the production and secretion of expressed GLP-1 by combining the advantages of signal peptide (SP) and two-step transcription amplification (TSTA) system. Its potential for GLP-1 gene delivery system was investigated with employment of arginine-grafted bioreducible polymer (ABP) as a gene carrier. Their polyplexes have about 140 nm-sizes and 20mV Zeta-potential values. ABP showed no cytotoxicity contrary to PEI25k. It was found in RT-PCR experiments that TSTA-SP pDNA systems showed increased GLP-1 gene transcription level in comparison withmono pDNA system(p beta-GLP-1). It was also observed in GLP-1 ELISA that GLP-1 secretion level of TSTA (SP-GLP-1) pDNA system was 2.7-3.4 times higher than those of p beta-GLP-1 and 1.5-1.7 times than TSTA (GLP-1). Additionally, 2.5-3.5 folds increased level of GLP-1 secretion was found in ABP gene carrier system in comparison with PEI25k. When transfection medium containing secreted GLP-1 was transferred to NIT-1 insulinoma cells, the highest secretion level of insulin was induced in ABP/TSTA (SP-GLP-1) polyplex medium-treated cells. Therefore, this novel system could be utilized as a safe and efficient GLP-1 gene delivery system for type 2 diabetes therapy. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER | - |
dc.title | Efficient GLP-1 gene delivery using two-step transcription amplification plasmid system with a secretion signal peptide and arginine-grafted bioreducible polymer | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Minhyung | - |
dc.identifier.doi | 10.1016/j.jconrel.2011.09.072 | - |
dc.identifier.scopusid | 2-s2.0-84855795348 | - |
dc.identifier.wosid | 000301045400010 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CONTROLLED RELEASE, v.157, no.2, pp.243 - 248 | - |
dc.relation.isPartOf | JOURNAL OF CONTROLLED RELEASE | - |
dc.citation.title | JOURNAL OF CONTROLLED RELEASE | - |
dc.citation.volume | 157 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 243 | - |
dc.citation.endPage | 248 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | GLUCAGON-LIKE PEPTIDE-1 | - |
dc.subject.keywordPlus | GASTRIC-INHIBITORY POLYPEPTIDE | - |
dc.subject.keywordPlus | FASTING HYPERGLYCEMIA | - |
dc.subject.keywordPlus | INSULIN-SECRETION | - |
dc.subject.keywordPlus | INCRETIN HORMONES | - |
dc.subject.keywordPlus | DIABETIC-PATIENTS | - |
dc.subject.keywordPlus | 7-36 AMIDE | - |
dc.subject.keywordPlus | GLUCOSE | - |
dc.subject.keywordPlus | TYPE-2 | - |
dc.subject.keywordPlus | CELL | - |
dc.subject.keywordAuthor | Gene delivery | - |
dc.subject.keywordAuthor | Glucagon-like peptide-1 | - |
dc.subject.keywordAuthor | Two-step transcription amplification | - |
dc.subject.keywordAuthor | Secretion signal peptide | - |
dc.subject.keywordAuthor | Arginine-grafted bioreducible polymer | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0168365911008571?via%3Dihub | - |
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