Evaluation of Double-Disk Potentiation and Disk Potentiation Tests Using Dipicolinic Acid for Detection of Metallo-beta-Lactamase-Producing Pseudomonas spp. and Acinetobacter spp.
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yong, Dongeun | - |
dc.contributor.author | Lee, Yang soon | - |
dc.contributor.author | Jeong, Seok Hoon | - |
dc.contributor.author | Lee, Kyungwon | - |
dc.contributor.author | Chong, Yunsop | - |
dc.date.accessioned | 2022-07-16T17:23:36Z | - |
dc.date.available | 2022-07-16T17:23:36Z | - |
dc.date.created | 2021-05-13 | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 0095-1137 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/166608 | - |
dc.description.abstract | Accurate detection of metallo-beta-lactamase (MBL)-producing Pseudomonas spp. and Acinetobacter spp. became very important with the increasing prevalence of carbapenem-nonsusceptible clinical isolates. The performance of phenotypic MBL detection methods may depend on the types of MBL and the characteristics of the isolates. A high false-positive rate is a problem with EDTA-based MBL detection methods. We evaluated the performance of double-disk potentiation tests (DDPTs) and disk potentiation tests (DPTs) with dipicolinic acid (DPA) using 44 isolates of Pseudomonas spp. and Acinetobacter spp. producing IMP-1-like, VIM-2-like, and SIM-1 type MBLs. Also, we characterized P. aeruginosa isolates with positive imipenem (IPM)-DPA DDPT, but negative meropenem (MEM)-DPA DDPT, and determined possibility of improving a DDPT by using MacConkey agar. Among five different DDPT methods, the IPM-DPA 250-mu g method showed the highest sensitivity (97.7%) and specificity (100%). Among four DPT tests, the highest sensitivity (100%) was shown by the IPM-EDTA 1,900-mu g disk method, but the specificity was very low (11.4%). Five of six P. aeruginosa isolates with false-negative DDPTs with MEM-DPA 250-mu g disks carried bla(IMP-6), and the high level resistance to MEM (MIC ˃= 512 mu g/ml) was reduced by the presence of phenylalanine arginine beta-naphtylamide. Improvement of DDPTs was observed when MacConkey agar was used instead of Mueller-Hinton agar. In conclusion, DPA is a better MBL inhibitor than EDTA for detection of Pseudomonas spp. and Acinetobacter spp. with IMP-1-like, VIM-2-like, and SIM-1-type MBLs. In DPA DDPTs, IPM disks perform better than MEM disks when the isolates are highly resistant to MEM due to the overexpression of efflux pumps. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | AMER SOC MICROBIOLOGY | - |
dc.title | Evaluation of Double-Disk Potentiation and Disk Potentiation Tests Using Dipicolinic Acid for Detection of Metallo-beta-Lactamase-Producing Pseudomonas spp. and Acinetobacter spp. | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Yang soon | - |
dc.identifier.doi | 10.1128/JCM.00818-12 | - |
dc.identifier.scopusid | 2-s2.0-84866437171 | - |
dc.identifier.wosid | 000308870200013 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CLINICAL MICROBIOLOGY, v.50, no.10, pp.3227 - 3232 | - |
dc.relation.isPartOf | JOURNAL OF CLINICAL MICROBIOLOGY | - |
dc.citation.title | JOURNAL OF CLINICAL MICROBIOLOGY | - |
dc.citation.volume | 50 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 3227 | - |
dc.citation.endPage | 3232 | - |
dc.type.rims | ART | - |
dc.type.docType | 정기학술지(Article(Perspective Article포함)) | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | SYNERGY TEST | - |
dc.subject.keywordPlus | HODGE TEST | - |
dc.subject.keywordPlus | AERUGINOSA | - |
dc.subject.keywordPlus | KOREA | - |
dc.subject.keywordPlus | CARBAPENEM | - |
dc.subject.keywordPlus | DISSEMINATION | - |
dc.subject.keywordPlus | BLA(SIM-1) | - |
dc.subject.keywordPlus | BAUMANNII | - |
dc.subject.keywordPlus | GENE | - |
dc.identifier.url | https://journals.asm.org/doi/10.1128/JCM.00818-12 | - |
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