Detection of Clostridium difficile toxin A/B genes by multiplex real-time PCR for the diagnosis of C. difficile infection
DC Field | Value | Language |
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dc.contributor.author | Kim, Heejung | - |
dc.contributor.author | Jeong, Seok Hoon | - |
dc.contributor.author | Kim, Myungsook | - |
dc.contributor.author | Lee, Yang soon | - |
dc.contributor.author | Lee, Kyungwon | - |
dc.date.accessioned | 2022-07-16T17:23:51Z | - |
dc.date.available | 2022-07-16T17:23:51Z | - |
dc.date.created | 2021-05-13 | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 0022-2615 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/166610 | - |
dc.description.abstract | Toxigenic Clostridium difficile culture is considered to be the standard diagnostic method for the detection of C. difficile infection (CDI). Culture methods are time-consuming and although enzyme immunoassay is rapid and easy to use, it has low sensitivity. In the present study, the AdvanSure CD real-time (RT)-PCR kit (LG Life Sciences) was evaluated for its ability to detect C. difficile toxin A (tcdA) and B (tcdB) genes, simultaneously. A total of 127 fresh diarrhoeal stool specimens, submitted to the clinical microbiology laboratory for C. difficile culture, were tested. C. difficile toxins and toxin genes were detected with a VIDAS C. difficile A&B (VIDAS-CDAB) enzyme-linked fluorescent immunoassay (ELFA) and the AdvanSure RT-PCR kit, respectively, according to the manufacturers’ instructions. Their performance was compared with a standard toxigenic culture method as a reference. The sensitivity, specificity and positive and negative predictive values using the AdvanSure RT-PCR kit were 100 %, 98.3 %, 84.6 % and 100 %, respectively, while those of the VIDAS-CDAB system were 63.6 %, 100 %, 100 % and 96.6 %, respectively. Four tcdA⁺/tcdB⁺ strains of C. difficile were detected with the AdvanSure RT-PCR kit, which offers comparable sensitivity and specificity to the reference method with a turnaround time of ~3 hours. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SOC GENERAL MICROBIOLOGY | - |
dc.title | Detection of Clostridium difficile toxin A/B genes by multiplex real-time PCR for the diagnosis of C. difficile infection | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Yang soon | - |
dc.identifier.doi | 10.1099/jmm.0.035618-0 | - |
dc.identifier.scopusid | 2-s2.0-84862928801 | - |
dc.identifier.wosid | 000300340500015 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MEDICAL MICROBIOLOGY, v.61, pp.274 - 277 | - |
dc.relation.isPartOf | JOURNAL OF MEDICAL MICROBIOLOGY | - |
dc.citation.title | JOURNAL OF MEDICAL MICROBIOLOGY | - |
dc.citation.volume | 61 | - |
dc.citation.startPage | 274 | - |
dc.citation.endPage | 277 | - |
dc.type.rims | ART | - |
dc.type.docType | 정기학술지(Article(Perspective Article포함)) | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | EPIDEMIOLOGY | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordPlus | SOCIETY | - |
dc.subject.keywordPlus | KOREA | - |
dc.subject.keywordPlus | TESTS | - |
dc.identifier.url | https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.035618-0 | - |
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