Fluazinam-induced apoptosis of SH-SY5Y cells is mediated by p53 and Bcl-2 family proteins
DC Field | Value | Language |
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dc.contributor.author | Lee, Jeong Eun | - |
dc.contributor.author | Kang, Jin Sun | - |
dc.contributor.author | Shin, In Chul | - |
dc.contributor.author | Lee, Soo-Jin | - |
dc.contributor.author | Hyun, Dong-Hoon | - |
dc.contributor.author | Lee, Kyung Suk | - |
dc.contributor.author | Koh, Hyun Chul | - |
dc.date.accessioned | 2022-07-16T17:50:21Z | - |
dc.date.available | 2022-07-16T17:50:21Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2011-12 | - |
dc.identifier.issn | 0161-813X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/166949 | - |
dc.description.abstract | A number of epidemiological studies have demonstrated a strong association between the incidence of neurodegenerative disease and pesticide exposure. Fluazinam (FZN) is a preventative fungicide from the pyridinamine group that was introduced in the 1990s and that quickly established itself as a new standard for the control of blight caused by Phytophthora infestans in potatoes. We used human neuroblastoma SH-SY5Y cells to investigate mechanisms of neuronal cell death in response to FZN and showed that FZN was cytotoxic to SH-SY5Y cells in a concentration- and time-dependent manner. Additionally, we showed that FZN treatment significantly decreased the neuron numbers including dopaminergic neurons and mitochondria] complex 1 activity. The cytotoxic effects of FZN were associated with an increase in reactive oxygen species (ROS) generation because pretreatment with N-acetyl cysteine, an anti-oxidant, reduced cell death. We showed that neuronal cell death in response to FZN was due to apoptosis because FZN increased cytochrome C release into the cytosol and activated caspase-3 through the accumulation of p53. FZN also reduced the levels of Bcl-2 protein but increased the levels of Bax. Our results provide insight into the molecular mechanisms of FZN-induced apoptosis in neuronal cells. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER | - |
dc.title | Fluazinam-induced apoptosis of SH-SY5Y cells is mediated by p53 and Bcl-2 family proteins | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Shin, In Chul | - |
dc.contributor.affiliatedAuthor | Lee, Soo-Jin | - |
dc.contributor.affiliatedAuthor | Koh, Hyun Chul | - |
dc.identifier.doi | 10.1016/j.neuro.2011.08.004 | - |
dc.identifier.scopusid | 2-s2.0-80055091982 | - |
dc.identifier.wosid | 000297000400003 | - |
dc.identifier.bibliographicCitation | NEUROTOXICOLOGY, v.32, no.6, pp.702 - 710 | - |
dc.relation.isPartOf | NEUROTOXICOLOGY | - |
dc.citation.title | NEUROTOXICOLOGY | - |
dc.citation.volume | 32 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 702 | - |
dc.citation.endPage | 710 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Neurosciences & Neurology | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalResearchArea | Toxicology | - |
dc.relation.journalWebOfScienceCategory | Neurosciences | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Toxicology | - |
dc.subject.keywordPlus | CYTOCHROME-C | - |
dc.subject.keywordPlus | NEURONAL APOPTOSIS | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | ORGANIC-COMPOUND | - |
dc.subject.keywordPlus | DEATH | - |
dc.subject.keywordPlus | MITOCHONDRIA | - |
dc.subject.keywordPlus | BAX | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | RELEASE | - |
dc.subject.keywordPlus | PESTICIDES | - |
dc.subject.keywordAuthor | Fluazinam | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | Reactive oxygen species | - |
dc.subject.keywordAuthor | p53 | - |
dc.subject.keywordAuthor | Bcl-2 family | - |
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