Combined delivery of dexamethasone and plasmid DNA in an animal model of LPS-induced acute lung injury
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Hyun Ah | - |
dc.contributor.author | Park, Ji Hwan | - |
dc.contributor.author | Lee, Sanghyun | - |
dc.contributor.author | Choi, Joon Sig | - |
dc.contributor.author | Rhim, Taiyoun | - |
dc.contributor.author | Lee, Minhyung | - |
dc.date.accessioned | 2022-07-16T18:25:37Z | - |
dc.date.available | 2022-07-16T18:25:37Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2011-11 | - |
dc.identifier.issn | 0168-3659 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/167267 | - |
dc.description.abstract | Dexamethasone was conjugated to low molecular weight polyethylenimine (2 kDa, PEI2k). Dexamethasone conjugated PEI2k (PEI2k-Dexa) was evaluated as a combined delivery carrier of dexamethasone and plasmid DNA (pDNA) in an animal model of lipopolysaccharide (LPS) induced acute lung injury (ALI). In vitro transfection of L2 lung epithelial cells, PEI2k-Dexa exhibited higher transfection efficiency than PEI2k or a simple mixture of PEI2k and dexamethasone. In addition, the PEI2k-Dexa/p beta-Luc complexes reduced the levels of pro-inflammatory cytokines in LPS activated Raw 264.7 macrophage cells. The anti-inflammatory effect of PEI2k-Dexa was higher than that of controls. The PEI2k-Dexa/p beta-Luc complexes were administered to mice via intratracheal injection. PEI2k-Dexa had higher pDNA delivery efficiency than PEI2k in the lung and decreased TNF-alpha and IL-6 in the lung homogenates and bronchoalveolar lavage (BAL) fluid compared with the controls. Furthermore, total protein and immunoglobulin M (IgM) concentrations in BAL fluid were reduced by the PEI2k-Dexa/p beta-Luc complexes. The intratracheal injection of the PEI2k-Dexa/pcDNA-EGFP complexes in the ALI model showed higher EGFP expression compared with PEI2k. Hematoxylin and eosin (H&E) staining showed that PEI2k-Dexa reduced inflammatory reaction in the lung. Therefore, PEI2k-Dexa may be useful for combination gene and drug therapy for ALI. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER | - |
dc.title | Combined delivery of dexamethasone and plasmid DNA in an animal model of LPS-induced acute lung injury | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Rhim, Taiyoun | - |
dc.contributor.affiliatedAuthor | Lee, Minhyung | - |
dc.identifier.doi | 10.1016/j.jconrel.2011.06.041 | - |
dc.identifier.scopusid | 2-s2.0-80855147570 | - |
dc.identifier.wosid | 000297928000009 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CONTROLLED RELEASE, v.156, no.1, pp.60 - 69 | - |
dc.relation.isPartOf | JOURNAL OF CONTROLLED RELEASE | - |
dc.citation.title | JOURNAL OF CONTROLLED RELEASE | - |
dc.citation.volume | 156 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 60 | - |
dc.citation.endPage | 69 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | WATER-SOLUBLE LIPOPOLYMER | - |
dc.subject.keywordPlus | GENE DELIVERY | - |
dc.subject.keywordPlus | CO-DELIVERY | - |
dc.subject.keywordPlus | BLOCK-COPOLYMER | - |
dc.subject.keywordPlus | POLYETHYLENIMINE | - |
dc.subject.keywordPlus | MICELLES | - |
dc.subject.keywordPlus | CARRIER | - |
dc.subject.keywordPlus | DRUG | - |
dc.subject.keywordPlus | PATHOGENESIS | - |
dc.subject.keywordPlus | ENDOTOXIN | - |
dc.subject.keywordAuthor | Gene transfer | - |
dc.subject.keywordAuthor | Gene therapy | - |
dc.subject.keywordAuthor | Drug delivery | - |
dc.subject.keywordAuthor | Lung | - |
dc.subject.keywordAuthor | Inflammation | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0168365911004767?via%3Dihub | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.