Prevention of free fatty acid-induced hepatic lipotoxicity by carnitine via reversal of mitochondrial dysfunction
DC Field | Value | Language |
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dc.contributor.author | Jun, Dae Won | - |
dc.contributor.author | Cho, Won Kyeong | - |
dc.contributor.author | Jun, Jin Hyun | - |
dc.contributor.author | Kwon, Hyuk Jin | - |
dc.contributor.author | Jang, Ki-Seok | - |
dc.contributor.author | Kim, Hyun-Jeong | - |
dc.contributor.author | Jeon, Hye Jun | - |
dc.contributor.author | Lee, Kang Nyeong | - |
dc.contributor.author | Lee, Hang Lak | - |
dc.contributor.author | Lee, Oh Young | - |
dc.contributor.author | Yoon, Byung Chul | - |
dc.contributor.author | Choi, Ho Soon | - |
dc.contributor.author | Hahm, Joon Soo | - |
dc.contributor.author | Lee, Min Ho | - |
dc.date.accessioned | 2022-07-16T18:51:17Z | - |
dc.date.available | 2022-07-16T18:51:17Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2011-10 | - |
dc.identifier.issn | 1478-3223 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/167465 | - |
dc.description.abstract | Background: Mitochondria are the main sites for fatty acid oxidation and play a central role in lipotoxicity and nonalcoholic steatohepatitis. Aims: We investigated whether carnitine prevents free fatty acid (FFA)-induced lipotoxicity in vitro and in vivo. Methods: HepG2 cells were incubated with FFA, along with carnitine and carnitine complexes. Mitochondrial beta-oxidation, transmembrane potential, intracellular ATP levels and changes in mitochondrial copy number and morphology were analysed. Otsuka Long-Evans Tokushima Fatty and Long-Evans Tokushima Otsuka rats were segregated into three experimental groups and fed for 8 weeks with (i) normal chow, (ii) a methionine choline-deficient (MCD) diet or (iii) an L-carnitine-supplemented MCD diet. Results: Carnitine prevented FFA-induced apoptosis (16% vs. 3%, P < 0.05). FFA treatment resulted in swollen mitochondria with increased inner matrix density and loss of cristae. However, mitochondria co-treated with carnitine had normal ultrastructure. The mitochondrial DNA copy number was higher in the carnitine treatment group than in the palmitic acid treatment group (375 vs. 221 copies, P < 0.05). The carnitine group showed higher mitochondrial beta-oxidation than did the control and palmitic acid treatment groups (597 vs. 432 and 395 ccpm, P < 0.05). Carnitine treatment increased the mRNA expression of carnitine palmitoyltransferase 1A and peroxisome proliferator-activated receptor-gamma, and carnitine-lipoic acid further augmented the mRNA expression. In the in vivo model, carnitine-treated rats showed lower alanine transaminase levels and lesser lobular inflammation than did the MCD-treated rats. Conclusions: Carnitine and carnitine-lipoic acid prevent lipotoxicity by increasing mitochondrial beta-oxidation and reducing intracellular oxidative stress. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.title | Prevention of free fatty acid-induced hepatic lipotoxicity by carnitine via reversal of mitochondrial dysfunction | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Jun, Dae Won | - |
dc.contributor.affiliatedAuthor | Jang, Ki-Seok | - |
dc.contributor.affiliatedAuthor | Lee, Hang Lak | - |
dc.contributor.affiliatedAuthor | Lee, Oh Young | - |
dc.contributor.affiliatedAuthor | Yoon, Byung Chul | - |
dc.contributor.affiliatedAuthor | Choi, Ho Soon | - |
dc.identifier.doi | 10.1111/j.1478-3231.2011.02602.x | - |
dc.identifier.scopusid | 2-s2.0-80052543705 | - |
dc.identifier.wosid | 000294822500009 | - |
dc.identifier.bibliographicCitation | LIVER INTERNATIONAL, v.31, no.9, pp.1315 - 1324 | - |
dc.relation.isPartOf | LIVER INTERNATIONAL | - |
dc.citation.title | LIVER INTERNATIONAL | - |
dc.citation.volume | 31 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 1315 | - |
dc.citation.endPage | 1324 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Gastroenterology & Hepatology | - |
dc.relation.journalWebOfScienceCategory | Gastroenterology & Hepatology | - |
dc.subject.keywordPlus | INSULIN-RESISTANCE | - |
dc.subject.keywordPlus | NONALCOHOLIC STEATOHEPATITIS | - |
dc.subject.keywordPlus | METABOLIC SYNDROME | - |
dc.subject.keywordPlus | BETA-OXIDATION | - |
dc.subject.keywordPlus | LIVER-DISEASE | - |
dc.subject.keywordPlus | DNA CONTENT | - |
dc.subject.keywordPlus | NASH | - |
dc.subject.keywordPlus | SUPPLEMENTATION | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | FATIGUE | - |
dc.subject.keywordAuthor | carnitine | - |
dc.subject.keywordAuthor | lipotoxicity | - |
dc.subject.keywordAuthor | mitochondria | - |
dc.identifier.url | https://onlinelibrary.wiley.com/doi/10.1111/j.1478-3231.2011.02602.x | - |
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