Recombinant human erythropoietin reduces aggregation of mutant Cu/Zn-binding superoxide dismutase (SOD1) in NSC-34 cells
DC Field | Value | Language |
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dc.contributor.author | Cho, Goang-Won | - |
dc.contributor.author | Kim, Ga-Young | - |
dc.contributor.author | Baek, Soojeong | - |
dc.contributor.author | Kim, Heejaung | - |
dc.contributor.author | Kim, Taikon | - |
dc.contributor.author | Kim, Hee Jin | - |
dc.contributor.author | Kim, Seung Hyun | - |
dc.date.accessioned | 2022-07-16T18:57:58Z | - |
dc.date.available | 2022-07-16T18:57:58Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2011-10 | - |
dc.identifier.issn | 0304-3940 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/167527 | - |
dc.description.abstract | Human erythropoietin (hEPO) has multiple actions in non-hematopoietic tissues, including neurotrophic, anti-oxidant, anti-apoptotic, and anti-inflammatory effects. To examine the effect of EPO in an vitro model of amyotrophic lateral sclerosis (ALS), we stably overexpressed wild SOD1 and a mutant form. SOD1/G93A, in NSC-34 motoneuron-like cells. Transformants harboring the wild and mutant forms of SOD1 were selected by G418 selection and immunoblot analysis. RT-PCR analysis showed that cox-2 expression was increased in the NSC-34/mSOD1s, and MU assays and BrdU-ELISAs revealed reduced cell growth and proliferation in the NSC-34/mSOD1 cell line. Incubation with 5 or 10 IU/mL rhEPO increased the viability and decreased the cox-2 expression in the dNSC-34/mSOD1s cells. Immunocytochemical staining with anti-SOD1 antibody revealed the presence of aggregates of mSOD1 protein in dNSC-34/mSOD1 cells. Incubation with10 IU/mL rhEPO reduced the proportion of cells containing such aggregates. Our findings suggest that the anti-oxidant and anti-inflammatory effects of EPO increase the survival of NSC-34/mSOD1 cells and reduce aggregation of the mutant SOD1 protein. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER IRELAND LTD | - |
dc.title | Recombinant human erythropoietin reduces aggregation of mutant Cu/Zn-binding superoxide dismutase (SOD1) in NSC-34 cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Hee Jin | - |
dc.contributor.affiliatedAuthor | Kim, Seung Hyun | - |
dc.identifier.doi | 10.1016/j.neulet.2011.09.008 | - |
dc.identifier.scopusid | 2-s2.0-80053994027 | - |
dc.identifier.wosid | 000297535000007 | - |
dc.identifier.bibliographicCitation | NEUROSCIENCE LETTERS, v.504, no.2, pp.107 - 111 | - |
dc.relation.isPartOf | NEUROSCIENCE LETTERS | - |
dc.citation.title | NEUROSCIENCE LETTERS | - |
dc.citation.volume | 504 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 107 | - |
dc.citation.endPage | 111 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Neurosciences & Neurology | - |
dc.relation.journalWebOfScienceCategory | Neurosciences | - |
dc.subject.keywordPlus | AMYOTROPHIC-LATERAL-SCLEROSIS | - |
dc.subject.keywordPlus | G93A-SOD1 MOUSE MODEL | - |
dc.subject.keywordPlus | MOTOR-NEURON DEATH | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | ALS | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordPlus | NEUROTOXICITY | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.subject.keywordAuthor | Erythropoietin | - |
dc.subject.keywordAuthor | ALS | - |
dc.subject.keywordAuthor | SOD1 | - |
dc.subject.keywordAuthor | Aggregation | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0304394011012730?via%3Dihub | - |
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