Apatite-Coated Collagen Scaffold for Bone Morphogenetic Protein-2 Delivery
- Authors
- Yang, Hee Seok; La, Wan-Geun; Bhang, Suk Ho; Lee, Tae-Jin; Lee, Minhyung; Kim, Byung-Soo
- Issue Date
- Sep-2011
- Publisher
- MARY ANN LIEBERT, INC
- Citation
- TISSUE ENGINEERING PART A, v.17, no.17-18, pp.2153 - 2164
- Indexed
- SCIE
SCOPUS
- Journal Title
- TISSUE ENGINEERING PART A
- Volume
- 17
- Number
- 17-18
- Start Page
- 2153
- End Page
- 2164
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/167660
- DOI
- 10.1089/ten.tea.2010.0702
- ISSN
- 1937-3341
- Abstract
- Bone morphogenetic proteins (BMPs) are the most potent osteoinductive growth factors. BMP-2 is clinically used for spine fusion and bone fracture healing. Commercially available BMP-2 uses a type I collagen scaffold as a carrier, but it only releases BMP-2 for a short period of time, which may release the bone formation efficacy. In the present study, we hypothesize that apatite coating of a collagen scaffold increases the release period as well as the osteogenic efficacy of BMP-2. Apatite coating was achieved by incubating collagen scaffolds in simulated body fluids (SBFs). Apatite coating on collagen scaffolds was confirmed by X-ray diffraction, electron spectroscopy for chemical analysis, attenuated total reflectance-Fourier transform infrared spectroscopy, and scanning electron microscopy. The rate and period of BMP-2 release from apatite-coated collagen scaffolds varied depending on the concentration of SBFs used. The 5x and 10x SBF apatite-coated collagen scaffolds released 91.8%+/- 11.5% and 82.2%+/- 13.1% of their loaded BMP-2 over 13 days in vitro, respectively, whereas noncoated collagen scaffold released 98.3%+/- 2.2% over the initial one day. BMP-2 released from apatite-coated collagen scaffold significantly increased the alkaline phosphatase activity of cultured osteoblasts, compared with BMP-2 released from noncoated collagen scaffold. Computed tomography and histomorphometry showed that BMP-2 delivery using apatite-coated collagen scaffolds resulted in 2.5-fold higher bone formation volume and 4.0-fold higher bone formation area than BMP-2 delivery using noncoated collagen scaffolds. This study shows that simple apatite coating of a collagen scaffold results in a BMP-2 carrier that renders long-term release of BMP-2 and dramatically enhances osteogenic efficacy.
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