The distinctive roles of erythroid specific activator GATA-1 and NF-E2 in transcription of the human fetal gamma-globin genes
DC Field | Value | Language |
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dc.contributor.author | Kim, Yea Woon | - |
dc.contributor.author | Kim, Seoyeon | - |
dc.contributor.author | Kim, Chul Geun | - |
dc.contributor.author | Kim, AeRi | - |
dc.date.accessioned | 2022-07-16T19:12:00Z | - |
dc.date.available | 2022-07-16T19:12:00Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2011-09 | - |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/167670 | - |
dc.description.abstract | GATA-1 and NF-E2 are erythroid specific activators that bind to the beta-globin locus. To explore the roles of these activators in transcription of the human fetal stage specific gamma-globin genes, we reduced GATA-1 and p45/NF-E2 using shRNA in erythroid K562 cells. GATA-1 or p45/NF-E2 knockdown inhibited the transcription of the gamma-globin genes, hypersensitive site (HS) formation in the LCR and chromatin loop formation of the beta-globin locus, but histone acetylation across the locus was decreased only in the case of GATA-1 knockdown. In p45/NF-E2 knockdown cells, GATA-1 binding was maintained at the LCR HSs and gamma-globin promoter, but NF-E2 binding at the LCR HSs was reduced by GATA-1 knockdown regardless of the amount of p45/NF-E2 in K562 cells. These results indicate that histone acetylation is dependent on GATA-1 binding, but the binding of GATA-1 is not sufficient for the gamma-globin transcription, HS formation and chromatin loop formation and NF-E2 is required. This idea is supported by the distinctive binding pattern of CBP and Brg1 in the beta-globin locus. Furthermore GATA-1-dependent loop formation between HS5 and 3'HS1 suggests correlation between histone modifications and chromatin looping. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.title | The distinctive roles of erythroid specific activator GATA-1 and NF-E2 in transcription of the human fetal gamma-globin genes | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Chul Geun | - |
dc.identifier.doi | 10.1093/nar/gkr253 | - |
dc.identifier.scopusid | 2-s2.0-80052429874 | - |
dc.identifier.wosid | 000294556800016 | - |
dc.identifier.bibliographicCitation | NUCLEIC ACIDS RESEARCH, v.39, no.16, pp.6944 - 6955 | - |
dc.relation.isPartOf | NUCLEIC ACIDS RESEARCH | - |
dc.citation.title | NUCLEIC ACIDS RESEARCH | - |
dc.citation.volume | 39 | - |
dc.citation.number | 16 | - |
dc.citation.startPage | 6944 | - |
dc.citation.endPage | 6955 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | LOCUS-CONTROL REGION | - |
dc.subject.keywordPlus | RNA-POLYMERASE-II | - |
dc.subject.keywordPlus | CHROMATIN-STRUCTURE | - |
dc.subject.keywordPlus | HISTONE ACETYLATION | - |
dc.subject.keywordPlus | QUANTITATIVE-ANALYSIS | - |
dc.subject.keywordPlus | HYPERSENSITIVE SITES | - |
dc.subject.keywordPlus | REGULATORY REGIONS | - |
dc.subject.keywordPlus | DELETION MUTATIONS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | MOUSE | - |
dc.identifier.url | https://academic.oup.com/nar/article/39/16/6944/2411224 | - |
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