Lung epithelial binding peptide-linked high mobility group box-1: A box for lung epithelial cell-specific delivery of DNA
DC Field | Value | Language |
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dc.contributor.author | Kim, Hyun Ah | - |
dc.contributor.author | Park, Ji Hwan | - |
dc.contributor.author | Cho, Su Hee | - |
dc.contributor.author | Lee, Minhyung | - |
dc.date.accessioned | 2022-07-16T19:40:38Z | - |
dc.date.available | 2022-07-16T19:40:38Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2011-08 | - |
dc.identifier.issn | 1061-186X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/167884 | - |
dc.description.abstract | High mobility group box-1 A box (HMGB1A) is an anti-inflammatory peptide originating from HMGB1. A previous report demonstrated that recombinant HMGB1A could deliver DNA into cells. Lung epithelial-specific gene delivery is required for the gene therapy of various lung diseases such as acute lung injury. In this study, a lung epithelial-specific DNA carrier was produced by linking the lung epithelial binding peptide (LEBP) to HMGB1A. An LEBP-linked HMGB1A (LEBP-HMGB1A) expression vector, pET21a-LEBP-HMGB1A, was constructed. LEBP-HMGB1A was expressed in BL21 strain and purified by consecutive applications of nickel affinity chromatography and cationic exchange chromatography. In a gel retardation assay, LEBP-HMGB1A completely retarded DNA at a 5:1 weight ratio (peptide: DNA). LEBP-HMGB1A/DNA complexes were prepared at various weight ratios, to which a fixed amount of polyethylenimine (2 kDa, PEI2k) was added to increase the proton buffering effect of the complex. LEBP-HMGB1A had the highest transfection efficiency to L2 lung epithelial cells at a 20: 1 weight ratio (peptide: DNA). At this ratio, LEBPHMGB1A had a higher transfection efficiency than poly-L-lysine (PLL) as well as HMGB1A without LEBP. A cytotoxicity assay showed that LEBP-HMGB1A was not toxic to L2 cells. Therefore, LEBP-HMGB1A may be useful in developing gene therapies for lung diseases. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | TAYLOR & FRANCIS LTD | - |
dc.title | Lung epithelial binding peptide-linked high mobility group box-1: A box for lung epithelial cell-specific delivery of DNA | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Minhyung | - |
dc.identifier.doi | 10.3109/1061186X.2010.547584 | - |
dc.identifier.scopusid | 2-s2.0-79960521622 | - |
dc.identifier.wosid | 000292841000012 | - |
dc.identifier.bibliographicCitation | JOURNAL OF DRUG TARGETING, v.19, no.7, pp.589 - 596 | - |
dc.relation.isPartOf | JOURNAL OF DRUG TARGETING | - |
dc.citation.title | JOURNAL OF DRUG TARGETING | - |
dc.citation.volume | 19 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 589 | - |
dc.citation.endPage | 596 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | LOW-MOLECULAR-WEIGHT | - |
dc.subject.keywordPlus | NUCLEIC-ACIDS | - |
dc.subject.keywordPlus | GENE-THERAPY | - |
dc.subject.keywordPlus | CYTOKINE | - |
dc.subject.keywordPlus | HMGB1 | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordPlus | HIGH-MOBILITY-GROUP-BOX-1 | - |
dc.subject.keywordPlus | CHROMOSOMAL-PROTEIN-1 | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordAuthor | Lung epithelial cells | - |
dc.subject.keywordAuthor | gene delivery | - |
dc.subject.keywordAuthor | high mobility group box-1 | - |
dc.subject.keywordAuthor | recombinant peptide | - |
dc.identifier.url | https://www.tandfonline.com/doi/full/10.3109/1061186X.2010.547584 | - |
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