Sphingosine 1-phosphate regulates matrix metalloproteinase-9 expression and breast cell invasion through S1P(3)-G(alpha q) coupling
DC Field | Value | Language |
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dc.contributor.author | Kim, Eun-Sook | - |
dc.contributor.author | Kim, Jong-Sook | - |
dc.contributor.author | Kim, Sang Geon | - |
dc.contributor.author | Hwang, Sejin | - |
dc.contributor.author | Lee, Chang Ho | - |
dc.contributor.author | Moon, Aree | - |
dc.date.accessioned | 2022-07-16T19:55:50Z | - |
dc.date.available | 2022-07-16T19:55:50Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2011-07 | - |
dc.identifier.issn | 0021-9533 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/168048 | - |
dc.description.abstract | Recent evidence suggests that inflammation is involved in malignant progression of breast cancer. Sphingosine 1-phosphate (S1P), acting on the G-protein-coupled receptors, is known as a potent inflammatory mediator. In this study, the effect of the inflammatory lipid S1P on the regulation of invasive/migratory phenotypes of MCF10A human breast epithelial cells was investigated to elucidate a causal relationship between inflammation and the control of invasiveness of breast cells. We show that S1P causes induction of matrix metalloproteinase-9 (MMP-9) in vitro and in vivo, and thus enhances invasion and migration. We also show that fos plays a crucial role in the transcriptional activation of MMP-9 by S1P. In addition, activation of extracellular-signal-regulated kinases 1 and 2 (ERK1/2), p38 and alpha serine/threonine-protein kinase (Akt) are involved in the process of S1P-mediated induction of MMP-9 expression and invasion. Activation of the S1P receptor S1P₃ and G(alpha q) are required for S1P-induced invasive/migratory responses, suggesting that the enhancement of S1P-mediated invasiveness is triggered by the specific coupling of S1P₃ to the heterotrimeric G(alpha q) subunit. Activation of phospholipase C-beta₄ and intracellular Ca²⁺ release are required for S1P-induced MMP-9 upregulation. Taken together, this study demonstrated that S1P regulates MMP-9 induction and invasiveness through coupling of S1P₃ and G(alpha q) in MCF10A cells, thus providing a molecular basis for the crucial role of S1P in promoting breast cell invasion. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | COMPANY BIOLOGISTS LTD | - |
dc.title | Sphingosine 1-phosphate regulates matrix metalloproteinase-9 expression and breast cell invasion through S1P(3)-G(alpha q) coupling | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Hwang, Sejin | - |
dc.contributor.affiliatedAuthor | Lee, Chang Ho | - |
dc.identifier.doi | 10.1242/jcs.076794 | - |
dc.identifier.scopusid | 2-s2.0-79960301614 | - |
dc.identifier.wosid | 000291537200012 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CELL SCIENCE, v.124, no.13, pp.2220 - 2230 | - |
dc.relation.isPartOf | JOURNAL OF CELL SCIENCE | - |
dc.citation.title | JOURNAL OF CELL SCIENCE | - |
dc.citation.volume | 124 | - |
dc.citation.number | 13 | - |
dc.citation.startPage | 2220 | - |
dc.citation.endPage | 2230 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | VASCULAR SMOOTH-MUSCLE | - |
dc.subject.keywordPlus | PHOSPHOLIPASE C-CA2+ SYSTEM | - |
dc.subject.keywordPlus | MIGRATION REQUIRES | - |
dc.subject.keywordPlus | UP-REGULATION | - |
dc.subject.keywordPlus | KAPPA-B | - |
dc.subject.keywordPlus | H-RAS | - |
dc.subject.keywordPlus | SPHINGOSINE-1-PHOSPHATE RECEPTORS | - |
dc.subject.keywordPlus | TRANSCRIPTIONAL ACTIVATION | - |
dc.subject.keywordPlus | IMMUNOSUPPRESSANT FTY720 | - |
dc.subject.keywordPlus | EPITHELIAL-CELLS | - |
dc.subject.keywordAuthor | S1P | - |
dc.subject.keywordAuthor | Breast cell invasion | - |
dc.subject.keywordAuthor | MMP-9 | - |
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