Enhanced chondrogenic marker expression of human mesenchymal stem cells by interaction with both TGF-beta 3 and hyaluronic acid
- Authors
- Bhang, Suk Ho; Jeon, Jeong-Yi; La, Wan-Geun; Seong, Jun Yeup; Hwang, Jin Wook; Ryu, Seong Eon; Kim, Byung-Soo
- Issue Date
- Jul-2011
- Publisher
- WILEY
- Keywords
- chondrogenesis; human mesenchymal stem cells; hyaluronic acid; transforming growth factor-beta3
- Citation
- BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, v.58, no.4, pp.271 - 276
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
- Volume
- 58
- Number
- 4
- Start Page
- 271
- End Page
- 276
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/168058
- DOI
- 10.1002/bab.39
- ISSN
- 0885-4513
- Abstract
- This study was designed to evaluate the additive effects of transforming growth factor-beta3 (TGF-beta 3) and hyaluronic acid (HA) on chondrogenic differentiation of human mesenchymal stem cells (hMSCs). The hMSCs were cultured on collagen type I-, HA-, or fibronectin-coated cell culture dishes with or without TGF-beta 3 added to the culture medium. Four weeks after cell culture, chondrogenic differentiation of hMSCs was determined by evaluating the expression of cartilage-specific markers using real-time polymerase chain reaction, immunocytochemistry, and Western blot analysis. hMSCs cultured on HA-coated dishes with TGF-beta 3 supplementation revealed a prominent increase in collagen type II, aggrecan, and Sox9. When hMSCs were cultured without TGF-beta 3 supplementation, only hMSCs cultured on HA-coated dishes showed prominent expression of the cartilage-specific markers. This study shows that chondrogenic differentiation of hMSCs can be enhanced additively by interactions with both a specific cell-adhesion matrix and a soluble growth factor.
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