Effect of hypoxia-inducible VEGF gene expression on revascularization and graft function in mouse islet transplantation
DC Field | Value | Language |
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dc.contributor.author | Lee, Byung Wan | - |
dc.contributor.author | Lee, Minhyung | - |
dc.contributor.author | Chae, Hee Young | - |
dc.contributor.author | Lee, Sanghyun | - |
dc.contributor.author | Kang, Jun Goo | - |
dc.contributor.author | Kim, Chul Sik | - |
dc.contributor.author | Lee, Seong Jin | - |
dc.contributor.author | Yoo, Hyung Joon | - |
dc.contributor.author | Ihm, Sung-Hee | - |
dc.date.accessioned | 2022-07-16T21:32:13Z | - |
dc.date.available | 2022-07-16T21:32:13Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2011-03 | - |
dc.identifier.issn | 0934-0874 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/168906 | - |
dc.description.abstract | P>For gene transfer strategies to improve islet engraftment, vascular endothelial growth factor (VEGF) expression should be regulated in a way that matches the transient nature of revascularization with simultaneously avoiding undesirable effects of overexpression. The aim of this study was to investigate the effects of hypoxia-inducible VEGF gene transfer using the RTP801 promoter on islet grafts. We implanted pSV-hVEGF transfected, pRTP801-hVEGF transfected or nontransfected mouse islets under the kidney capsule of streptozotocin-induced diabetic syngeneic mice. Human VEGF immunostaining of day 3 grafts revealed that the pRTP801-hVEGF transfected group had higher hVEGF expression compared with the pSV-hVEGF transfected group. BS-1 staining of day 3 grafts from the pRTP801-hVEGF transfected group showed the highest vascular density, which was comparable with day 6 grafts from the nontransfected group. In 360 islet equivalent (IEQ)-transplantation which reverted hyperglycemia in all mice, the area under the curve of glucose levels during intraperitoneal glucose tolerance test 7 weeks post-transplant was lower in mice transplanted with pRTP801-hVEGF transfected grafts compared with mice transplanted with nontransfected grafts. In 220 IEQ-transplantations, diabetic mice transplanted with pRTP801-hVEGF islets became normoglycemic more rapidly compared with mice transplanted with pSV-hVEGF or nontransfected islets, and diabetes reversal rate after 50 days was 90%, 68%, and 50%, respectively. In conclusion, our results indicate that regulated overexpression of hVEGF in a hypoxia-inducible manner enhances islet vascular engraftment and preserves islet function overtime in transplants. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.title | Effect of hypoxia-inducible VEGF gene expression on revascularization and graft function in mouse islet transplantation | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Minhyung | - |
dc.identifier.doi | 10.1111/j.1432-2277.2010.01194.x | - |
dc.identifier.scopusid | 2-s2.0-79551542032 | - |
dc.identifier.wosid | 000286838700013 | - |
dc.identifier.bibliographicCitation | TRANSPLANT INTERNATIONAL, v.24, no.3, pp.307 - 314 | - |
dc.relation.isPartOf | TRANSPLANT INTERNATIONAL | - |
dc.citation.title | TRANSPLANT INTERNATIONAL | - |
dc.citation.volume | 24 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 307 | - |
dc.citation.endPage | 314 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Surgery | - |
dc.relation.journalResearchArea | Transplantation | - |
dc.relation.journalWebOfScienceCategory | Surgery | - |
dc.relation.journalWebOfScienceCategory | Transplantation | - |
dc.subject.keywordPlus | GROWTH-FACTOR PRODUCTION | - |
dc.subject.keywordPlus | HUMAN PANCREATIC-ISLETS | - |
dc.subject.keywordPlus | RTP801 PROMOTER | - |
dc.subject.keywordPlus | RAT ISLETS | - |
dc.subject.keywordPlus | FOLLOW-UP | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | ANGIOGENESIS | - |
dc.subject.keywordPlus | ENGRAFTMENT | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordAuthor | hypoxia | - |
dc.subject.keywordAuthor | islet | - |
dc.subject.keywordAuthor | RTP801 | - |
dc.subject.keywordAuthor | transplantation | - |
dc.subject.keywordAuthor | vascular endothelial growth factor | - |
dc.identifier.url | https://onlinelibrary.wiley.com/doi/10.1111/j.1432-2277.2010.01194.x | - |
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