T-cell responses to versican in ankylosing spondylitis
DC Field | Value | Language |
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dc.contributor.author | Kim, Tae-Jong | - |
dc.contributor.author | Kim, Tae-Hwan | - |
dc.contributor.author | Lee, Hyun-Joo | - |
dc.contributor.author | Lee, Bitnara | - |
dc.contributor.author | Poole, A. Robin | - |
dc.contributor.author | Inman, Robert D. | - |
dc.date.accessioned | 2022-07-16T21:48:10Z | - |
dc.date.available | 2022-07-16T21:48:10Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2011-02 | - |
dc.identifier.issn | 0172-8172 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/169083 | - |
dc.description.abstract | The objective of our study was to undertake a systematic analysis of the T-cell response to the proteoglycan versican G1-globular domain (VG1) in ankylosing spondylitis (AS) as immunity to VG1 in mice can induce a pathology closely resembling AS. Peripheral blood lymphocytes from 36 AS patients and 33 healthy controls were incubated with recombinant human VG1 in culture for 6 h. T-cell responses were assessed by FACS analyses using mAb against surface expression of the activation marker CD69 and against the intracellular cytokines interferon (IFN)-gamma, tumor necrosis factor (TNF)-alpha. T cells activated by exposure to versican were determined by assessing the percentage of CD4+ or CD8+ T cells that were CD69/cytokine double-positive cells as compared to isotype control staining. In the AS patients, exposure to VG1 resulted in increased expression by CD4+ T cells of IFN-gamma in 55.6% of patients and of TNF-alpha in 52.8% of patients. In the controls, only 36.4% of subjects demonstrated an IFN-gamma response and 36.4% demonstrated a TNF-alpha response (P value 0.148, 0.227, respectively). With respect to CD8+ T-cell responses, versican stimulation enhanced IFN-gamma expression in 44.4% of AS patients and 39.4% of controls, and enhanced TNF-alpha response in 50.0% of AS patients and 39.4% of controls (P value 0.620, 0.327, respectively). Although, there was no statistically significant difference in the magnitude of the IFN-gamma or TNF secretion by CD4+ T cells and CD8+ T cells between AS and controls, our results demonstrate an enhanced T-cell response to VG1 in AS. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER HEIDELBERG | - |
dc.title | T-cell responses to versican in ankylosing spondylitis | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Tae-Hwan | - |
dc.identifier.doi | 10.1007/s00296-009-1248-1 | - |
dc.identifier.scopusid | 2-s2.0-79954447428 | - |
dc.identifier.wosid | 000286466500008 | - |
dc.identifier.bibliographicCitation | RHEUMATOLOGY INTERNATIONAL, v.31, no.2, pp.191 - 195 | - |
dc.relation.isPartOf | RHEUMATOLOGY INTERNATIONAL | - |
dc.citation.title | RHEUMATOLOGY INTERNATIONAL | - |
dc.citation.volume | 31 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 191 | - |
dc.citation.endPage | 195 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Rheumatology | - |
dc.relation.journalWebOfScienceCategory | Rheumatology | - |
dc.subject.keywordPlus | BALB/C MICE | - |
dc.subject.keywordPlus | RHEUMATOID-ARTHRITIS | - |
dc.subject.keywordPlus | CARTILAGE PROTEOGLYCANS | - |
dc.subject.keywordPlus | CYTOKINE PRODUCTION | - |
dc.subject.keywordPlus | KERATAN SULFATE | - |
dc.subject.keywordPlus | FLOW-CYTOMETRY | - |
dc.subject.keywordPlus | WHOLE-BLOOD | - |
dc.subject.keywordPlus | G1 DOMAIN | - |
dc.subject.keywordPlus | IMMUNITY | - |
dc.subject.keywordPlus | ANTIGEN | - |
dc.subject.keywordAuthor | Ankylosing spondylitis | - |
dc.subject.keywordAuthor | T cell | - |
dc.subject.keywordAuthor | Proteoglycan | - |
dc.subject.keywordAuthor | Versican | - |
dc.identifier.url | https://link.springer.com/article/10.1007%2Fs00296-009-1248-1 | - |
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