Altered Expression of Tight Junction Proteins in Cyclosporine Nephrotoxicity
DC Field | Value | Language |
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dc.contributor.author | Lee, Chang Hwa | - |
dc.contributor.author | Kim, Sua | - |
dc.contributor.author | Kang, Chong Myung | - |
dc.contributor.author | Kim, Wan Young | - |
dc.contributor.author | Kim, Jin | - |
dc.contributor.author | Kim, Gheun-Ho | - |
dc.date.accessioned | 2022-07-16T22:34:38Z | - |
dc.date.available | 2022-07-16T22:34:38Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 0250-8095 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/169419 | - |
dc.description.abstract | Background/Aims: The increased permeability of chloride in the distal cortical nephron in cyclosporine nephrotoxicity may involve the transcellular pathway mediated by the thiazide-sensitive Na+-Cl- cotransporter and/or the paracellular pathway mediated by the tight junctions (TJs). Methods: Cyclosporine was subcutaneously administered to Sprague-Dawley rats for 6 (7.5 mg/kg body weight) and 2 (25 mg/kg body weight) weeks, and immunoblot analysis and immunohistochemistry were carried out from the kidneys. Electrically tight epithelial Madin-Darby canine kidney (MDCK) I cells were exposed to cyclosporine for 72 h to measure changes in transepithelial electrical resistance (Delta TER). Results: Cyclosporine treatment induced a decrease in Na+-Cl- cotransporter in rat renal cortex. WNK4 protein was increased in both rat kidneys and MDCK I cells. Occludin was also increased in rat kidneys and MDCK I cells exposed to 100 ng/ml cyclosporine. In contrast, cyclosporine treatment induced a decrease in zonula occludens 1 protein abundance and no changes in claudin-1 and claudin-4 in both rat kidneys and MDCK I cells. As a measure of the barrier to small ions, Delta TER of MDCK monolayers was decreased by 100 ng/ml cyclosporine. Conclusion: Renal TJ proteins are affected by cyclosporine treatment. Changes in TJ protein assembly induced by altered expression of WNK4, occludin, and zonula occludens 1 may affect paracellular permeability. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KARGER | - |
dc.title | Altered Expression of Tight Junction Proteins in Cyclosporine Nephrotoxicity | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Chang Hwa | - |
dc.contributor.affiliatedAuthor | Kim, Gheun-Ho | - |
dc.identifier.doi | 10.1159/000322445 | - |
dc.identifier.scopusid | 2-s2.0-78649517125 | - |
dc.identifier.wosid | 000286570300002 | - |
dc.identifier.bibliographicCitation | AMERICAN JOURNAL OF NEPHROLOGY, v.33, no.1, pp.7 - 16 | - |
dc.relation.isPartOf | AMERICAN JOURNAL OF NEPHROLOGY | - |
dc.citation.title | AMERICAN JOURNAL OF NEPHROLOGY | - |
dc.citation.volume | 33 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 7 | - |
dc.citation.endPage | 16 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Urology & Nephrology | - |
dc.relation.journalWebOfScienceCategory | Urology & Nephrology | - |
dc.subject.keywordPlus | EPITHELIAL BARRIER FUNCTION | - |
dc.subject.keywordPlus | GLOMERULAR-FILTRATION-RATE | - |
dc.subject.keywordPlus | THICK ASCENDING LIMB | - |
dc.subject.keywordPlus | NA-CL COTRANSPORTER | - |
dc.subject.keywordPlus | LONG-TERM TREATMENT | - |
dc.subject.keywordPlus | WNK KINASES | - |
dc.subject.keywordPlus | PARACELLULAR PERMEABILITY | - |
dc.subject.keywordPlus | CHLORIDE REABSORPTION | - |
dc.subject.keywordPlus | POTASSIUM-TRANSPORT | - |
dc.subject.keywordPlus | MUTANT WNK4 | - |
dc.subject.keywordAuthor | Cyclosporine nephrotoxicity | - |
dc.subject.keywordAuthor | Paracellular permeability | - |
dc.subject.keywordAuthor | Sodium chloride symporter | - |
dc.subject.keywordAuthor | Tight junction proteins | - |
dc.subject.keywordAuthor | WNK4 protein | - |
dc.identifier.url | https://www.karger.com/Article/FullText/322445 | - |
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