Effect of Mortalin on Scar Formation in Human Dermal Fibroblasts and a Rat Incisional Scar Model
DC Field | Value | Language |
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dc.contributor.author | Jung, Bok Ki | - |
dc.contributor.author | Roh, Tai Suk | - |
dc.contributor.author | Roh, Hyun | - |
dc.contributor.author | Lee, Ju Hee | - |
dc.contributor.author | Yun, Chae-Ok | - |
dc.contributor.author | Lee, Won Jai | - |
dc.date.accessioned | 2022-09-19T13:36:47Z | - |
dc.date.available | 2022-09-19T13:36:47Z | - |
dc.date.created | 2022-09-08 | - |
dc.date.issued | 2022-07 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/171576 | - |
dc.description.abstract | Wound healing is a complicated cascading process; disequilibrium among reparative processes leads to the formation of pathologic scars. Herein, we explored the role of mortalin in scar formation and its association with the interleukin-1 alpha receptor using in vitro and in vivo models. To investigate the effects of mortalin, we performed an MTT cell viability assay, qRT-PCR, and Western blot analyses, in addition to immunofluorescence and immunoprecipitation studies using cultured fibroblasts. A rat incisional wound model was used to evaluate the effect of a mortalin-specific shRNA (dE1-RGD/GFP/shMot) Ad vector in scar tissue. In vitro, the mortalin-treated human dermal fibroblast displayed a significant increase in proliferation of type I collagen, alpha-smooth muscle actin, transforming growth factor-beta, phospho-Smad2/3-complex, and NF-kappa B levels. Immunofluorescence staining revealed markedly increased mortalin and interleukin-1 alpha receptor protein in keloid tissue compared to those in normal tissue, suggesting that the association between mortalin and IL-1 alpha receptor was responsible for the fibrogenic effect. In vivo, mortalin-specific shRNA-expressing Ad vectors significantly decreased the scar size and type-I-collagen, alpha-SMA, and phospho-Smad2/3-complex expression in rat incisional scar tissue. Thus, dE1-RGD/GEP/shMot can inhibit the TGF-beta/alpha-SMA axis and NF-kappa B signal pathways in scar formation, and blocking endogenous mortalin could be a potential therapeutic target for keloids. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.title | Effect of Mortalin on Scar Formation in Human Dermal Fibroblasts and a Rat Incisional Scar Model | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Yun, Chae-Ok | - |
dc.identifier.doi | 10.3390/ijms23147918 | - |
dc.identifier.scopusid | 2-s2.0-85135116748 | - |
dc.identifier.wosid | 000831660700001 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.23, no.14, pp.1 - 17 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.title | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.volume | 23 | - |
dc.citation.number | 14 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 17 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | ONCOLYTIC ADENOVIRUS | - |
dc.subject.keywordPlus | HYPERTROPHIC SCAR | - |
dc.subject.keywordPlus | GROWTH-FACTOR | - |
dc.subject.keywordPlus | ALGINATE GEL | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | KELOIDS | - |
dc.subject.keywordPlus | RNA | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordAuthor | scar | - |
dc.subject.keywordAuthor | keloid | - |
dc.subject.keywordAuthor | mortalin | - |
dc.subject.keywordAuthor | adenovirus | - |
dc.subject.keywordAuthor | interleukin-1 alpha receptor | - |
dc.subject.keywordAuthor | fibrogenesis | - |
dc.identifier.url | https://www.mdpi.com/1422-0067/23/14/7918 | - |
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