Injectable, thermo-reversible and complex coacervated combination gels containing IL-1 receptor antagonis inhibits IL-1 beta-induced MMP-3 expression in human osteoarthritis chondrocytes
DC Field | Value | Language |
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dc.contributor.author | Jun, Jae-Bum | - |
dc.contributor.author | Na, Young-In | - |
dc.contributor.author | Choi, Choong Hyeok | - |
dc.contributor.author | Kim, Jang Kyoung | - |
dc.contributor.author | Kim, Yong-Hee | - |
dc.date.accessioned | 2022-10-07T10:03:51Z | - |
dc.date.available | 2022-10-07T10:03:51Z | - |
dc.date.created | 2022-08-26 | - |
dc.date.issued | 2008-09 | - |
dc.identifier.issn | 1043-4666 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/171871 | - |
dc.description.abstract | IL-1 has been implicated in degradation of articular cartilage in various arthritides including osteoarthritis (OA). Therefore, blocking of IL-1 by IL-1 receptor antagonist (IL-1Ra) may be the pathogenesis-based strategy for inhibiting degradation of cartilage matrix. We investigated the role of injectable, thermo-reversible and complex coacervated combination gels containing IL-1Ra for matrix degradation in OA.The combination gels, injectable to the joint, were formed in aqueous solution by preparing complex coacervate with IL-1Ra and cationic macromolecules, and co-formulating with methylcellulose. The gels containing IL-1Ra were positioned in upper insert of transwell system, and human OA chondrocytes were placed in lower compartment and stimulated with IL-1β. Expression of matrix metalloproteinase 3 (MMP-3) was examined by real time PCR and ELISA.Release profile of IL-1Ra from the gels showed a sustained release pattern through 6 days with minimal initial bursts. IL-1Ra-loaded gels after 5 days of formation were used. IL-1b markedly induced the expression of MMP-3 gene and protein. However, loading of combination gels containing IL-1Ra profoundly inhibited IL-1β-induced MMP-3 expression in human OA chondrocytes. We are planning to examine the effectiveness of 30 day-matured gels in degradation of extracellular matrix with articular chondrocytes and cartilage explants.We applied our optimized novel in situ gel depot system containing IL-1Ra to human OA chondrocytes in transwell system. According to our results, intraarticular injection of our gels containing IL-1Ra into OA knee will provide a prolonged therapeutic option based on the pathophysiology in the treatment of knee OA. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD | - |
dc.title | Injectable, thermo-reversible and complex coacervated combination gels containing IL-1 receptor antagonis inhibits IL-1 beta-induced MMP-3 expression in human osteoarthritis chondrocytes | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Jun, Jae-Bum | - |
dc.contributor.affiliatedAuthor | Choi, Choong Hyeok | - |
dc.contributor.affiliatedAuthor | Kim, Yong-Hee | - |
dc.identifier.doi | 10.1016/j.cyto.2008.07.137 | - |
dc.identifier.wosid | 000260212900115 | - |
dc.identifier.bibliographicCitation | CYTOKINE, v.43, no.3, pp.258 - 258 | - |
dc.relation.isPartOf | CYTOKINE | - |
dc.citation.title | CYTOKINE | - |
dc.citation.volume | 43 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 258 | - |
dc.citation.endPage | 258 | - |
dc.type.rims | ART | - |
dc.type.docType | Meeting Abstract | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S1043466608003505?via%3Dihub | - |
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