Effective suppression of HIV-1 by artificial bispecific miRNA targeting conserved sequences with tolerance for wobble base-pairing
DC Field | Value | Language |
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dc.contributor.author | Son, Jiyeon | - |
dc.contributor.author | Uchil, Pradeep D. | - |
dc.contributor.author | Kim, Young Bong | - |
dc.contributor.author | Shankar, Premlata | - |
dc.contributor.author | Kumar, Priti | - |
dc.contributor.author | Lee, Sang-Kyung | - |
dc.date.accessioned | 2022-10-07T10:06:31Z | - |
dc.date.available | 2022-10-07T10:06:31Z | - |
dc.date.created | 2022-08-26 | - |
dc.date.issued | 2008-09 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/171892 | - |
dc.description.abstract | The high genetic diversity and mutability of HIV pose a major problem for RNAi-mediated antiviral therapy. Simultaneous targeting of multiple highly conserved viral sequences has been suggested for durable cross-clade inhibition. Here we validate the approach of co-targeting two conserved sequences in the Tat and Vif genes. When coexpressed as artificial microRNA from a PoIII driven miR-155-based vector, the sequences together mediated effective and Sustained inhibition of HIV replication without virus break-out. To understand the nature of this efficient control, we analyzed genome sequences of 625 HIV-1 isolates in the Los Alamos Sequence database. Interestingly most natural variants were capable of wobble binding with the Tat/Vif siRNAs. Efficient silencing of reporter luciferase constructs bearing these variants residues verified that the Tat/Vif sequences together tolerated wobble binding and mediated functional RNAi. We propose the rationale of targeting highly conserved HIV sequences where wobble substitutions permit functional RNAi for global HIV repression. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | Effective suppression of HIV-1 by artificial bispecific miRNA targeting conserved sequences with tolerance for wobble base-pairing | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Sang-Kyung | - |
dc.identifier.doi | 10.1016/j.bbrc.2008.06.125 | - |
dc.identifier.scopusid | 2-s2.0-50949086562 | - |
dc.identifier.wosid | 000258600100008 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.374, no.2, pp.214 - 218 | - |
dc.relation.isPartOf | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.title | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.volume | 374 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 214 | - |
dc.citation.endPage | 218 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.subject.keywordPlus | RNA INTERFERENCE | - |
dc.subject.keywordPlus | SIRNA | - |
dc.subject.keywordPlus | ESCAPE | - |
dc.subject.keywordAuthor | RNA interference | - |
dc.subject.keywordAuthor | HIV | - |
dc.subject.keywordAuthor | siRNA | - |
dc.subject.keywordAuthor | shRNA | - |
dc.subject.keywordAuthor | miRNA | - |
dc.subject.keywordAuthor | conserved | - |
dc.subject.keywordAuthor | lentivirus | - |
dc.subject.keywordAuthor | wobble | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0006291X08013028?via%3Dihub | - |
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