Expression, purification and characterization of TAT-high mobility group box-1A peptide as a carrier of nucleic acids
DC Field | Value | Language |
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dc.contributor.author | Kim, Kyunghwa | - |
dc.contributor.author | Han, Jee Seung | - |
dc.contributor.author | Kim, Hyun Ah | - |
dc.contributor.author | Lee, Minhyung | - |
dc.date.accessioned | 2022-10-07T10:09:30Z | - |
dc.date.available | 2022-10-07T10:09:30Z | - |
dc.date.created | 2022-08-26 | - |
dc.date.issued | 2008-08 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/171922 | - |
dc.description.abstract | High mobility group box 1 (HMGB1) is an abundant nuclear protein that binds to double-stranded DNA. HMGB1 is composed of high mobility (HMG) box A, box B, and C-terminal acidic regions. In this study, a recombinant TAT linked HMGB1 box A (rTAT-HMGB1A) peptide was expressed, purified, and characterized as a carrier of nucleic acids. The HMGB1A cDNA was amplified by PCR, and cloned into the pET21a expression vector with the TAT domain located at the N-terminus. The rTAT-HMGB1A peptide was overexpressed and purified using Nickel affinity chromatography. A recombinant HMGB1A (rHMGB1A) peptide without the TAT domain was also overexpressed and purified as a control. In gel retardation assays, both the rHMGB1A and rTAT-HMGB1A peptides formed complexes with DNA equally well. However, transfection assays showed that the rTAT-HMGB1A peptide had a higher gene transfer efficiency than rHMGB1A. Finally, rTAT-HMGB1A had no cytotoxicity to HEK 293 cells suggesting that rTAT-HMGB1A may be useful as a non-toxic gene delivery carrier. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER | - |
dc.title | Expression, purification and characterization of TAT-high mobility group box-1A peptide as a carrier of nucleic acids | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Minhyung | - |
dc.identifier.doi | 10.1007/s10529-008-9695-4 | - |
dc.identifier.scopusid | 2-s2.0-45849092575 | - |
dc.identifier.wosid | 000256909200004 | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY LETTERS, v.30, no.8, pp.1331 - 1337 | - |
dc.relation.isPartOf | BIOTECHNOLOGY LETTERS | - |
dc.citation.title | BIOTECHNOLOGY LETTERS | - |
dc.citation.volume | 30 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 1331 | - |
dc.citation.endPage | 1337 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | NONVIRAL GENE DELIVERY | - |
dc.subject.keywordPlus | PLASMID DNA DELIVERY | - |
dc.subject.keywordPlus | LOW-MOLECULAR-WEIGHT | - |
dc.subject.keywordPlus | CYTOKINE | - |
dc.subject.keywordPlus | HMGB1 | - |
dc.subject.keywordAuthor | gene delivery | - |
dc.subject.keywordAuthor | high mobility group box 1 | - |
dc.subject.keywordAuthor | peptide | - |
dc.subject.keywordAuthor | purification | - |
dc.subject.keywordAuthor | transfection | - |
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